Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques
© The Institution of Engineering and Technology 2017. p-Hydroxyphenylacetate 3-hydroxylase component 1 (C 1 ) is a useful enzyme for generating reduced flavin and NAD + intermediates. In this study, poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were used to encapsulate the C 1 (PLGA-C 1 NPs...
Saved in:
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2019
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/45153 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.45153 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.451532019-08-23T18:27:08Z Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques Komkrich Sawasdee Jeerus Sucharitakul Taweesak Dhammaraj Nuttawee Niamsiri Pimchai Chaiyen Kanlaya Prapainop Vidyasirimedhi Institute of Science and Technology Chulalongkorn University Mahidol University Mahasarakham University Biochemistry, Genetics and Molecular Biology Engineering Materials Science © The Institution of Engineering and Technology 2017. p-Hydroxyphenylacetate 3-hydroxylase component 1 (C 1 ) is a useful enzyme for generating reduced flavin and NAD + intermediates. In this study, poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were used to encapsulate the C 1 (PLGA-C 1 NPs). Enzymatic activity, stability, and reusability of PLGA-C 1 NPs prepared using three different methods [oil in water (o/w), water in oil in water (w/o/w), and solid in oil in water (s/o/w)] were compared. The s/o/w provided the optimal conditions for encapsulation of C 1 (PLGA-C 1,s NPs), giving the highest enzyme activity, stability, and reusability. The s/o/w method improves enzyme activity ∼11 and 9-fold compared to w/o/w (PLGA-C 1,w NPs) and o/w (PLGA-C 1,o NPs). In addition, s/o/w prepared PLGA-C 1,s NPs could be reused 14 times with nearly 50% activity remaining, a much higher reusability compared to PLGA-C 1,o NPs and PLGA-C 1,w NPs. These nanovesicles were successfully utilised to generate reduced flavin mononucleotide (FMN) and supply this cofactor to a hydroxylase enzyme that has application for synthesising anti-inflammatory compounds. Therefore, this recycling biocatalyst prepared using the s/o/w method is effective and has the potential for use in combination with other enzymes that require reduced FMN. Application of PLGA-C 1,s NPs may be possible in additional biocatalytic processes for chemical or biochemical production. 2019-08-23T10:32:53Z 2019-08-23T10:32:53Z 2018-06-01 Article IET Nanobiotechnology. Vol.12, No.4 (2018), 423-428 10.1049/iet-nbt.2017.0189 17518741 2-s2.0-85047336169 https://repository.li.mahidol.ac.th/handle/123456789/45153 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85047336169&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology Engineering Materials Science |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Engineering Materials Science Komkrich Sawasdee Jeerus Sucharitakul Taweesak Dhammaraj Nuttawee Niamsiri Pimchai Chaiyen Kanlaya Prapainop Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
description |
© The Institution of Engineering and Technology 2017. p-Hydroxyphenylacetate 3-hydroxylase component 1 (C 1 ) is a useful enzyme for generating reduced flavin and NAD + intermediates. In this study, poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were used to encapsulate the C 1 (PLGA-C 1 NPs). Enzymatic activity, stability, and reusability of PLGA-C 1 NPs prepared using three different methods [oil in water (o/w), water in oil in water (w/o/w), and solid in oil in water (s/o/w)] were compared. The s/o/w provided the optimal conditions for encapsulation of C 1 (PLGA-C 1,s NPs), giving the highest enzyme activity, stability, and reusability. The s/o/w method improves enzyme activity ∼11 and 9-fold compared to w/o/w (PLGA-C 1,w NPs) and o/w (PLGA-C 1,o NPs). In addition, s/o/w prepared PLGA-C 1,s NPs could be reused 14 times with nearly 50% activity remaining, a much higher reusability compared to PLGA-C 1,o NPs and PLGA-C 1,w NPs. These nanovesicles were successfully utilised to generate reduced flavin mononucleotide (FMN) and supply this cofactor to a hydroxylase enzyme that has application for synthesising anti-inflammatory compounds. Therefore, this recycling biocatalyst prepared using the s/o/w method is effective and has the potential for use in combination with other enzymes that require reduced FMN. Application of PLGA-C 1,s NPs may be possible in additional biocatalytic processes for chemical or biochemical production. |
author2 |
Vidyasirimedhi Institute of Science and Technology |
author_facet |
Vidyasirimedhi Institute of Science and Technology Komkrich Sawasdee Jeerus Sucharitakul Taweesak Dhammaraj Nuttawee Niamsiri Pimchai Chaiyen Kanlaya Prapainop |
format |
Article |
author |
Komkrich Sawasdee Jeerus Sucharitakul Taweesak Dhammaraj Nuttawee Niamsiri Pimchai Chaiyen Kanlaya Prapainop |
author_sort |
Komkrich Sawasdee |
title |
Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
title_short |
Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
title_full |
Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
title_fullStr |
Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
title_full_unstemmed |
Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
title_sort |
encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques |
publishDate |
2019 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/45153 |
_version_ |
1763489656902516736 |