In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate
© 2018 The Author(s). Background: Technical limitations for culturing the human malaria parasite Plasmodium vivax have impaired the discovery of vaccine candidates, challenging the malaria eradication agenda. The immunogenicity of the M2 domain of the Merozoite Adhesive Erythrocytic Binding Protein...
Saved in:
Main Authors: | , , , , , , , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2019
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/46057 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.46057 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.460572019-08-28T13:27:18Z In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate Pedro Cravo Renato B. Machado Juliana A. Leite Taizy Leda Rossarin Suwanarusk Najara Bittencourt Letusa Albrecht Carla Judice Stefanie C.P. Lopes Marcus V.G. Lacerda Marcelo U. Ferreira Irene S. Soares Yun Shan Goh Daniel Y. Bargieri François Nosten Bruce Russell Laurent Rénia Fabio T.M. Costa A-Star, Singapore Immunology Network Fundacao de Medicina Tropical do Amazonas Universidade Nova de Lisboa, Instituto de Higiene e Medicina Tropical Universidade Estadual de Campinas Universidade Federal de Goias Fundacao Oswaldo Cruz National University of Singapore Mahidol University Nuffield Department of Clinical Medicine Universidade de Sao Paulo - USP PPG-SOMA Immunology and Microbiology Medicine © 2018 The Author(s). Background: Technical limitations for culturing the human malaria parasite Plasmodium vivax have impaired the discovery of vaccine candidates, challenging the malaria eradication agenda. The immunogenicity of the M2 domain of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) antigen cloned from the Plasmodium yoelii murine parasite, has been previously demonstrated. Results: Detailed epitope mapping of MAEBL through immunoinformatics identified several MHCI, MHCII and B cell epitopes throughout the peptide, with several of these lying in the M2 domain and being conserved between P. vivax, P. yoelii and Plasmodium falciparum, hinting that the M2-MAEBL is pan-reactive. This hypothesis was tested through functional assays, showing that P. yoelii M2-MAEBL antisera are able to recognize and inhibit erythrocyte invasion from both P. falciparum and P. vivax parasites isolated from Thai patients, in ex vivo assays. Moreover, the sequence of the M2-MAEBL is shown to be highly conserved between P. vivax isolates from the Amazon and Thailand, indicating that the MAEBL antigen may constitute a vaccine candidate outwitting strain-specific immunity. Conclusions: The MAEBL antigen is promising candidate towards the development of a malaria vaccine. 2019-08-23T11:23:24Z 2019-08-23T11:23:24Z 2018-01-10 Article Malaria Journal. Vol.17, No.1 (2018) 10.1186/s12936-017-2144-x 14752875 2-s2.0-85043785897 https://repository.li.mahidol.ac.th/handle/123456789/46057 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85043785897&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Immunology and Microbiology Medicine |
spellingShingle |
Immunology and Microbiology Medicine Pedro Cravo Renato B. Machado Juliana A. Leite Taizy Leda Rossarin Suwanarusk Najara Bittencourt Letusa Albrecht Carla Judice Stefanie C.P. Lopes Marcus V.G. Lacerda Marcelo U. Ferreira Irene S. Soares Yun Shan Goh Daniel Y. Bargieri François Nosten Bruce Russell Laurent Rénia Fabio T.M. Costa In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate |
description |
© 2018 The Author(s). Background: Technical limitations for culturing the human malaria parasite Plasmodium vivax have impaired the discovery of vaccine candidates, challenging the malaria eradication agenda. The immunogenicity of the M2 domain of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) antigen cloned from the Plasmodium yoelii murine parasite, has been previously demonstrated. Results: Detailed epitope mapping of MAEBL through immunoinformatics identified several MHCI, MHCII and B cell epitopes throughout the peptide, with several of these lying in the M2 domain and being conserved between P. vivax, P. yoelii and Plasmodium falciparum, hinting that the M2-MAEBL is pan-reactive. This hypothesis was tested through functional assays, showing that P. yoelii M2-MAEBL antisera are able to recognize and inhibit erythrocyte invasion from both P. falciparum and P. vivax parasites isolated from Thai patients, in ex vivo assays. Moreover, the sequence of the M2-MAEBL is shown to be highly conserved between P. vivax isolates from the Amazon and Thailand, indicating that the MAEBL antigen may constitute a vaccine candidate outwitting strain-specific immunity. Conclusions: The MAEBL antigen is promising candidate towards the development of a malaria vaccine. |
author2 |
A-Star, Singapore Immunology Network |
author_facet |
A-Star, Singapore Immunology Network Pedro Cravo Renato B. Machado Juliana A. Leite Taizy Leda Rossarin Suwanarusk Najara Bittencourt Letusa Albrecht Carla Judice Stefanie C.P. Lopes Marcus V.G. Lacerda Marcelo U. Ferreira Irene S. Soares Yun Shan Goh Daniel Y. Bargieri François Nosten Bruce Russell Laurent Rénia Fabio T.M. Costa |
format |
Article |
author |
Pedro Cravo Renato B. Machado Juliana A. Leite Taizy Leda Rossarin Suwanarusk Najara Bittencourt Letusa Albrecht Carla Judice Stefanie C.P. Lopes Marcus V.G. Lacerda Marcelo U. Ferreira Irene S. Soares Yun Shan Goh Daniel Y. Bargieri François Nosten Bruce Russell Laurent Rénia Fabio T.M. Costa |
author_sort |
Pedro Cravo |
title |
In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate |
title_short |
In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate |
title_full |
In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate |
title_fullStr |
In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate |
title_full_unstemmed |
In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate |
title_sort |
in silico epitope mapping and experimental evaluation of the merozoite adhesive erythrocytic binding protein (maebl) as a malaria vaccine candidate |
publishDate |
2019 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/46057 |
_version_ |
1763492400900079616 |