Differential contribution of pyruvate carboxylation to anaplerosis and cataplerosis during non-gluconeogenic and gluconeogenic conditions in HepG2 cells

• Main Authors: Siriluck Wattanavanitchakorn, Israr H. Ansari, Mahmoud El Azzouny, Melissa J. Longacre, Scott W. Stoker, Michael J. MacDonald, Sarawut Jitrapakdee
• Other Authors: University of Wisconsin School of Medicine and Public Health
• Format: Article
• Published: 2020
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/50033
• Institution: Mahidol University

© 2019 Elsevier Inc. Pyruvate carboxylase (PC) is an anaplerotic enzyme that supplies oxaloacetate to mitochondria enabling the maintenance of other metabolic intermediates consumed by cataplerosis. Using liquid chromatography mass spectrometry (LC-MS) to measure metabolic intermediates derived from...