Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
© 2019, © 2019 Taylor & Francis Group, LLC. 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes o...
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th-mahidol.501082020-01-27T14:40:33Z Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization Ninticha Thavoncharoensub Kento Maruyama Choon Han Heh Kok Hoong Leong Hui Shi Yoshiharu Shigematsu Shigeki Sasaki Yosuke Taniguchi University of Malaya Mahidol University Kyushu University Biochemistry, Genetics and Molecular Biology © 2019, © 2019 Taylor & Francis Group, LLC. 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase. 2020-01-27T07:40:33Z 2020-01-27T07:40:33Z 2019-08-03 Article Nucleosides, Nucleotides and Nucleic Acids. Vol.38, No.8 (2019), 578-589 10.1080/15257770.2019.1586919 15322335 15257770 2-s2.0-85063631756 https://repository.li.mahidol.ac.th/handle/123456789/50108 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063631756&origin=inward |
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Biochemistry, Genetics and Molecular Biology Ninticha Thavoncharoensub Kento Maruyama Choon Han Heh Kok Hoong Leong Hui Shi Yoshiharu Shigematsu Shigeki Sasaki Yosuke Taniguchi Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
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© 2019, © 2019 Taylor & Francis Group, LLC. 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase. |
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University of Malaya |
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University of Malaya Ninticha Thavoncharoensub Kento Maruyama Choon Han Heh Kok Hoong Leong Hui Shi Yoshiharu Shigematsu Shigeki Sasaki Yosuke Taniguchi |
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Article |
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Ninticha Thavoncharoensub Kento Maruyama Choon Han Heh Kok Hoong Leong Hui Shi Yoshiharu Shigematsu Shigeki Sasaki Yosuke Taniguchi |
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Ninticha Thavoncharoensub |
title |
Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
title_short |
Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
title_full |
Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
title_fullStr |
Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
title_full_unstemmed |
Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
title_sort |
synthesis of γ-n-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization |
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2020 |
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https://repository.li.mahidol.ac.th/handle/123456789/50108 |
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1763497244274720768 |