Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization

© 2019, © 2019 Taylor & Francis Group, LLC. 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes o...

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Main Authors: Ninticha Thavoncharoensub, Kento Maruyama, Choon Han Heh, Kok Hoong Leong, Hui Shi, Yoshiharu Shigematsu, Shigeki Sasaki, Yosuke Taniguchi
Other Authors: University of Malaya
Format: Article
Published: 2020
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/50108
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spelling th-mahidol.501082020-01-27T14:40:33Z Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization Ninticha Thavoncharoensub Kento Maruyama Choon Han Heh Kok Hoong Leong Hui Shi Yoshiharu Shigematsu Shigeki Sasaki Yosuke Taniguchi University of Malaya Mahidol University Kyushu University Biochemistry, Genetics and Molecular Biology © 2019, © 2019 Taylor & Francis Group, LLC. 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase. 2020-01-27T07:40:33Z 2020-01-27T07:40:33Z 2019-08-03 Article Nucleosides, Nucleotides and Nucleic Acids. Vol.38, No.8 (2019), 578-589 10.1080/15257770.2019.1586919 15322335 15257770 2-s2.0-85063631756 https://repository.li.mahidol.ac.th/handle/123456789/50108 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063631756&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Ninticha Thavoncharoensub
Kento Maruyama
Choon Han Heh
Kok Hoong Leong
Hui Shi
Yoshiharu Shigematsu
Shigeki Sasaki
Yosuke Taniguchi
Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
description © 2019, © 2019 Taylor & Francis Group, LLC. 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase.
author2 University of Malaya
author_facet University of Malaya
Ninticha Thavoncharoensub
Kento Maruyama
Choon Han Heh
Kok Hoong Leong
Hui Shi
Yoshiharu Shigematsu
Shigeki Sasaki
Yosuke Taniguchi
format Article
author Ninticha Thavoncharoensub
Kento Maruyama
Choon Han Heh
Kok Hoong Leong
Hui Shi
Yoshiharu Shigematsu
Shigeki Sasaki
Yosuke Taniguchi
author_sort Ninticha Thavoncharoensub
title Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
title_short Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
title_full Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
title_fullStr Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
title_full_unstemmed Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
title_sort synthesis of γ-n-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
publishDate 2020
url https://repository.li.mahidol.ac.th/handle/123456789/50108
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