Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

• Main Authors: Wannaporn Ittiprasert, Victoria H. Mann, Shannon E. Karinshak, Avril Coghlan, Gabriel Rinaldi, Geetha Sankaranarayanan, Apisit Chaidee, Toshihiko Tanno, Chutima Kumkhaek, Pannathee Prangtaworn, Margaret M. Mentink-Kane, Christina J. Cochran, Patrick Driguez, Nancy Holroyd, Alan Tracey, Rutchanee Rodpai, Bart Everts, Cornelis H. Hokke, Karl F. Hoffmann, Matthew Berriman, Paul J. Brindley
• Other Authors: Institute of Biological, Environmental and Rural Sciences
• Format: Article
• Published: 2020
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/50291
• Institution: Mahidol University

CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma mansoni as proof of principle. This secreted ribonuclease is crucial for Th2 polarization and granuloma formation. Schistosome eggs were exposed...