RNA-Guided AsCas12a-and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni

RNA-Guided AsCas12a-and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni

The efficiency of the RNA-guided AsCas12a nuclease of Acidaminococcus sp. was compared with SpCas9 from Streptococcus pyogenes, for functional genomics in Schistosoma mansoni. We deployed optimized conditions for the ratio of guide RNAs to the nuclease, donor templates, and electroporation parameter...

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Bibliographic Details
Main Authors: Wannaporn Ittiprasert, Chawalit Chatupheeraphat, Victoria H. Mann, Wenhui Li, André Miller, Taiwo Ogunbayo, Kenny Tran, Yousef N. Alrefaei, Margaret Mentink-Kane, Paul J. Brindley
Other Authors: The George Washington University School of Medicine and Health Sciences
Format: Article
Published: 2022
Subjects:
Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Computer Science
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/73534
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Institution: Mahidol University

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https://repository.li.mahidol.ac.th/handle/123456789/73534

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