Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial s...
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th-mahidol.577272020-08-25T16:06:00Z Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli‐guivarc’h Christophe Hano Bilal Haider Abbasi Biomolécules et Biotechnologies Végétales (BBV) CRMHT Centre de Recherches sur les Matériaux à Haute Température Quaid-i-Azam University Universite d'Orleans Mahidol University Virtual University of Pakistan Kinnaird College for Women Biochemistry, Genetics and Molecular Biology © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem‐derived callus of L. usitatissimum. Seedlings of L. usitatissimum grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol‐β‐coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1‐ naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP‐treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP‐treated callus. In both systems, the dose‐dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures. 2020-08-25T09:06:00Z 2020-08-25T09:06:00Z 2020-06-01 Article Biomolecules. Vol.10, No.6 (2020), 1-16 10.3390/biom10060918 2218273X 2-s2.0-85086659145 https://repository.li.mahidol.ac.th/handle/123456789/57727 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85086659145&origin=inward |
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Biochemistry, Genetics and Molecular Biology Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli‐guivarc’h Christophe Hano Bilal Haider Abbasi Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis |
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem‐derived callus of L. usitatissimum. Seedlings of L. usitatissimum grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol‐β‐coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1‐ naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP‐treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP‐treated callus. In both systems, the dose‐dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures. |
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Biomolécules et Biotechnologies Végétales (BBV) |
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Biomolécules et Biotechnologies Végétales (BBV) Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli‐guivarc’h Christophe Hano Bilal Haider Abbasi |
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Article |
author |
Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli‐guivarc’h Christophe Hano Bilal Haider Abbasi |
author_sort |
Afifa Zaeem |
title |
Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis |
title_short |
Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis |
title_full |
Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis |
title_fullStr |
Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis |
title_full_unstemmed |
Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis |
title_sort |
effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. in vitro cultures: a comparative analysis |
publishDate |
2020 |
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https://repository.li.mahidol.ac.th/handle/123456789/57727 |
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1763489012027228160 |