Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR

• Main Authors: F. Haque, S. Fisseha, P. Athamanolap, R. Tower, J. Ortega, C. Dominguez, T. Maruca, D. Torpey, R. Myers, P. Laksanalamai
• Other Authors: Mahidol University
• Format: Article
• Published: 2020
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/59857

© 2020 Elsevier B.V. Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6–24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.