Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians
© 2020 American Academy of Allergy, Asthma & Immunology Background: Co-trimoxazole, a sulfonamide antibiotic, is used to treat a variety of infections worldwide, and it remains a common first-line medicine for prophylaxis against Pneumocystis jiroveci pneumonia. However, it can cause severe cu...
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th-mahidol.600012022-06-02T10:29:21Z Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians Chuang Wei Wang Wichittra Tassaneeyakul Chun Bing Chen Wei Ti Chen Yu Chuan Teng Cheng Yang Huang Chonlaphat Sukasem Chun Wei Lu Yun Shien Lee Siew Eng Choon Nontaya Nakkam Rosaline Chung Yee Hui Yen Hua Huang Ya Ching Chang Yang Yu Wei Lin Chee Jen Chang Tsu Man Chiu Wasun Chantratita Parinya Konyoung Chaw Ning Lee Jettanong Klaewsongkram Ticha Rerkpattanapipat Warayuwadee Amornpinyo Niwat Saksit Pawinee Rerknimitr Yu Huei Huang Shang Hung Lin Chao Kai Hsu Cheng Chi Chan Yu Lin Shuen Iu Hung Wen Hung Chung Xiamen Chang Gung Hospital Chang Gung University College of Medicine National Cheng Kung University Hospital National Yang-Ming University Taiwan Chang Gung Memorial Hospital Udon Thani Center Hospital Chung Yuan Christian University Ming Chuan University Chulalongkorn University Chung Shan Medical University Tsinghua University Shanghai Jiao Tong University King Chulalongkorn Memorial Hospital, Faculty of Medicine Chulalongkorn University Changhua Christian Hospital Taiwan Chang Gung University Monash University Malaysia Khon Kaen University Faculty of Medicine, Ramathibodi Hospital, Mahidol University National Cheng Kung University Khon Kaen Regional Hospital Thai Severe Cutaneous Adverse Drug Reaction Research Group Immunology and Microbiology Medicine © 2020 American Academy of Allergy, Asthma & Immunology Background: Co-trimoxazole, a sulfonamide antibiotic, is used to treat a variety of infections worldwide, and it remains a common first-line medicine for prophylaxis against Pneumocystis jiroveci pneumonia. However, it can cause severe cutaneous adverse reaction (SCAR), including Stevens-Johnson syndrome, toxic epidermal necrolysis, and drug reaction with eosinophilia and systemic symptoms. The pathomechanism of co-trimoxazole–induced SCAR remains unclear. Objective: We aimed to investigate the genetic predisposition of co-trimoxazole–induced SCAR. Methods: We conducted a multicountry case-control association study that included 151 patients with of co-trimoxazole–induced SCAR and 4631 population controls from Taiwan, Thailand, and Malaysia, as well as 138 tolerant controls from Taiwan. Whole-genome sequencing was performed for the patients and population controls from Taiwan; it further validated the results from Thailand and Malaysia. Results: The whole-genome sequencing study (43 case patients vs 507 controls) discovered that the single-nucleotide polymorphism rs41554616, which is located between the HLA-B and MICA loci, had the strongest association with co-trimoxazole–induced SCAR (P = 8.2 × 10−9; odds ratio [OR] = 7.7). There were weak associations of variants in co-trimoxazole–related metabolizing enzymes (CYP2D6, GSTP1, GCLC, N-acetyltransferase [NAT2], and CYP2C8). A replication study using HLA genotyping revealed that HLA-B∗13:01 was strongly associated with co-trimoxazole–induced SCAR (the combined sample comprised 91 case patients vs 2545 controls [P = 7.2 × 10−21; OR = 8.7]). A strong HLA association was also observed in the case patients from Thailand (P = 3.2 × 10−5; OR = 3.6) and Malaysia (P = .002; OR = 12.8), respectively. A meta-analysis and phenotype stratification study further indicated a strong association between HLA-B∗13:01 and co-trimoxazole–induced drug reaction with eosinophilia and systemic symptoms (P = 4.2 × 10−23; OR = 40.1). Conclusion: This study identified HLA-B∗13:01 as an important genetic factor associated with co-trimoxazole–induced SCAR in Asians. 2020-11-18T09:41:59Z 2020-11-18T09:41:59Z 2020-01-01 Article Journal of Allergy and Clinical Immunology. (2020) 10.1016/j.jaci.2020.08.003 10976825 00916749 2-s2.0-85092363324 https://repository.li.mahidol.ac.th/handle/123456789/60001 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85092363324&origin=inward |
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Immunology and Microbiology Medicine Chuang Wei Wang Wichittra Tassaneeyakul Chun Bing Chen Wei Ti Chen Yu Chuan Teng Cheng Yang Huang Chonlaphat Sukasem Chun Wei Lu Yun Shien Lee Siew Eng Choon Nontaya Nakkam Rosaline Chung Yee Hui Yen Hua Huang Ya Ching Chang Yang Yu Wei Lin Chee Jen Chang Tsu Man Chiu Wasun Chantratita Parinya Konyoung Chaw Ning Lee Jettanong Klaewsongkram Ticha Rerkpattanapipat Warayuwadee Amornpinyo Niwat Saksit Pawinee Rerknimitr Yu Huei Huang Shang Hung Lin Chao Kai Hsu Cheng Chi Chan Yu Lin Shuen Iu Hung Wen Hung Chung Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians |
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© 2020 American Academy of Allergy, Asthma & Immunology Background: Co-trimoxazole, a sulfonamide antibiotic, is used to treat a variety of infections worldwide, and it remains a common first-line medicine for prophylaxis against Pneumocystis jiroveci pneumonia. However, it can cause severe cutaneous adverse reaction (SCAR), including Stevens-Johnson syndrome, toxic epidermal necrolysis, and drug reaction with eosinophilia and systemic symptoms. The pathomechanism of co-trimoxazole–induced SCAR remains unclear. Objective: We aimed to investigate the genetic predisposition of co-trimoxazole–induced SCAR. Methods: We conducted a multicountry case-control association study that included 151 patients with of co-trimoxazole–induced SCAR and 4631 population controls from Taiwan, Thailand, and Malaysia, as well as 138 tolerant controls from Taiwan. Whole-genome sequencing was performed for the patients and population controls from Taiwan; it further validated the results from Thailand and Malaysia. Results: The whole-genome sequencing study (43 case patients vs 507 controls) discovered that the single-nucleotide polymorphism rs41554616, which is located between the HLA-B and MICA loci, had the strongest association with co-trimoxazole–induced SCAR (P = 8.2 × 10−9; odds ratio [OR] = 7.7). There were weak associations of variants in co-trimoxazole–related metabolizing enzymes (CYP2D6, GSTP1, GCLC, N-acetyltransferase [NAT2], and CYP2C8). A replication study using HLA genotyping revealed that HLA-B∗13:01 was strongly associated with co-trimoxazole–induced SCAR (the combined sample comprised 91 case patients vs 2545 controls [P = 7.2 × 10−21; OR = 8.7]). A strong HLA association was also observed in the case patients from Thailand (P = 3.2 × 10−5; OR = 3.6) and Malaysia (P = .002; OR = 12.8), respectively. A meta-analysis and phenotype stratification study further indicated a strong association between HLA-B∗13:01 and co-trimoxazole–induced drug reaction with eosinophilia and systemic symptoms (P = 4.2 × 10−23; OR = 40.1). Conclusion: This study identified HLA-B∗13:01 as an important genetic factor associated with co-trimoxazole–induced SCAR in Asians. |
author2 |
Xiamen Chang Gung Hospital |
author_facet |
Xiamen Chang Gung Hospital Chuang Wei Wang Wichittra Tassaneeyakul Chun Bing Chen Wei Ti Chen Yu Chuan Teng Cheng Yang Huang Chonlaphat Sukasem Chun Wei Lu Yun Shien Lee Siew Eng Choon Nontaya Nakkam Rosaline Chung Yee Hui Yen Hua Huang Ya Ching Chang Yang Yu Wei Lin Chee Jen Chang Tsu Man Chiu Wasun Chantratita Parinya Konyoung Chaw Ning Lee Jettanong Klaewsongkram Ticha Rerkpattanapipat Warayuwadee Amornpinyo Niwat Saksit Pawinee Rerknimitr Yu Huei Huang Shang Hung Lin Chao Kai Hsu Cheng Chi Chan Yu Lin Shuen Iu Hung Wen Hung Chung |
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Article |
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Chuang Wei Wang Wichittra Tassaneeyakul Chun Bing Chen Wei Ti Chen Yu Chuan Teng Cheng Yang Huang Chonlaphat Sukasem Chun Wei Lu Yun Shien Lee Siew Eng Choon Nontaya Nakkam Rosaline Chung Yee Hui Yen Hua Huang Ya Ching Chang Yang Yu Wei Lin Chee Jen Chang Tsu Man Chiu Wasun Chantratita Parinya Konyoung Chaw Ning Lee Jettanong Klaewsongkram Ticha Rerkpattanapipat Warayuwadee Amornpinyo Niwat Saksit Pawinee Rerknimitr Yu Huei Huang Shang Hung Lin Chao Kai Hsu Cheng Chi Chan Yu Lin Shuen Iu Hung Wen Hung Chung |
author_sort |
Chuang Wei Wang |
title |
Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians |
title_short |
Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians |
title_full |
Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians |
title_fullStr |
Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians |
title_full_unstemmed |
Whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in Asians |
title_sort |
whole genome sequencing identifies genetic variants associated with co-trimoxazole hypersensitivity in asians |
publishDate |
2020 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/60001 |
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1763495438859632640 |