Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency

Cationic lipids are widely used as nonviral synthetic vectors for gene delivery as a safer alternative to viral vectors. In this work, a library of L-shaped spermine-based cationic lipids with identical and nonidentical hydrophobic chains having variable carbon lengths (from C10 to C18) was designed...

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Main Authors: Chopaka Thongbamrer, Wanlapa Roobsoong, Jetsumon Sattabongkot, Praneet Opanasopit, Boon ek Yingyongnarongkul
Other Authors: Faculty of Tropical Medicine, Mahidol University
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Published: 2022
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/73388
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spelling th-mahidol.733882022-08-04T10:51:23Z Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency Chopaka Thongbamrer Wanlapa Roobsoong Jetsumon Sattabongkot Praneet Opanasopit Boon ek Yingyongnarongkul Faculty of Tropical Medicine, Mahidol University Ramkhamhaeng University Silpakorn University Biochemistry, Genetics and Molecular Biology Chemistry Cationic lipids are widely used as nonviral synthetic vectors for gene delivery as a safer alternative to viral vectors. In this work, a library of L-shaped spermine-based cationic lipids with identical and nonidentical hydrophobic chains having variable carbon lengths (from C10 to C18) was designed and synthesized. These lipids were characterized and the structure-activity relationships of these compounds were determined for DNA binding and transfection ability when formulated as cationic liposomes. The liposomes were then used successfully for the transfection of HEK293T, HeLa, PC3, H460, HepG2, SH-SY5Y and Calu’3 cell lines. The transfection efficiency of lipids with nonidentical hydrocarbon chains was greater than the identical analogue. These reagents exhibited superior efficiency to the commercial reagent, Lipofectamine3000, under both serum-free and 10–40 % serum conditions in HEK293T, HeLa and H460 cell lines. The lipids were not toxic to the tested cell line. The results suggest that L-shaped spermine-based cationic lipids with nonidentical hydrocarbon tails could serve as efficient and safe nonviral vector gene carriers in further in vivo studies. 2022-08-04T03:42:36Z 2022-08-04T03:42:36Z 2022-03-18 Article ChemBioChem. Vol.23, No.6 (2022) 10.1002/cbic.202100672 14397633 14394227 2-s2.0-85123750250 https://repository.li.mahidol.ac.th/handle/123456789/73388 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85123750250&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Chopaka Thongbamrer
Wanlapa Roobsoong
Jetsumon Sattabongkot
Praneet Opanasopit
Boon ek Yingyongnarongkul
Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency
description Cationic lipids are widely used as nonviral synthetic vectors for gene delivery as a safer alternative to viral vectors. In this work, a library of L-shaped spermine-based cationic lipids with identical and nonidentical hydrophobic chains having variable carbon lengths (from C10 to C18) was designed and synthesized. These lipids were characterized and the structure-activity relationships of these compounds were determined for DNA binding and transfection ability when formulated as cationic liposomes. The liposomes were then used successfully for the transfection of HEK293T, HeLa, PC3, H460, HepG2, SH-SY5Y and Calu’3 cell lines. The transfection efficiency of lipids with nonidentical hydrocarbon chains was greater than the identical analogue. These reagents exhibited superior efficiency to the commercial reagent, Lipofectamine3000, under both serum-free and 10–40 % serum conditions in HEK293T, HeLa and H460 cell lines. The lipids were not toxic to the tested cell line. The results suggest that L-shaped spermine-based cationic lipids with nonidentical hydrocarbon tails could serve as efficient and safe nonviral vector gene carriers in further in vivo studies.
author2 Faculty of Tropical Medicine, Mahidol University
author_facet Faculty of Tropical Medicine, Mahidol University
Chopaka Thongbamrer
Wanlapa Roobsoong
Jetsumon Sattabongkot
Praneet Opanasopit
Boon ek Yingyongnarongkul
format Article
author Chopaka Thongbamrer
Wanlapa Roobsoong
Jetsumon Sattabongkot
Praneet Opanasopit
Boon ek Yingyongnarongkul
author_sort Chopaka Thongbamrer
title Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency
title_short Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency
title_full Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency
title_fullStr Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency
title_full_unstemmed Serum Compatible Spermine-Based Cationic Lipids with Nonidentical Hydrocarbon Tails Mediate High Transfection Efficiency
title_sort serum compatible spermine-based cationic lipids with nonidentical hydrocarbon tails mediate high transfection efficiency
publishDate 2022
url https://repository.li.mahidol.ac.th/handle/123456789/73388
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