RNA-Guided AsCas12a-and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni

• Main Authors: Wannaporn Ittiprasert, Chawalit Chatupheeraphat, Victoria H. Mann, Wenhui Li, André Miller, Taiwo Ogunbayo, Kenny Tran, Yousef N. Alrefaei, Margaret Mentink-Kane, Paul J. Brindley
• 其他作者: The George Washington University School of Medicine and Health Sciences
• 格式: Article
• 出版: 2022
• 主題: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Chemistry; Computer Science
• 在線閱讀: https://repository.li.mahidol.ac.th/handle/123456789/73534
• 機構: Mahidol University

The efficiency of the RNA-guided AsCas12a nuclease of Acidaminococcus sp. was compared with SpCas9 from Streptococcus pyogenes, for functional genomics in Schistosoma mansoni. We deployed optimized conditions for the ratio of guide RNAs to the nuclease, donor templates, and electroporation parameter...