The role of the phosphate groups of trinitrophenyl adenosine 5′-triphosphate (TNP-ATP) in allosteric activation of pyruvate carboxylase and the inhibition of acetyl CoA-dependent activation

• Main Authors: Khanti Rattanapornsompong, Chaiyos Sirithanakorn, Sarawut Jitrapakdee, Paul V. Attwood
• Other Authors: The University of Western Australia
• Format: Article
• Published: 2022
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/75993
• Institution: Mahidol University

A previous study showed that 2′-3′-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate (TNP-ATP) was a weak allosteric activator of Rhizobium etli pyruvate carboxylase (RePC) in the absence of acetyl-CoA. On the other hand, TNP-ATP inhibited the allosteric activation of RePC by acetyl-CoA. Here, we a...