Determination of Chinese hamster ovary (CHO) cell densities and antibody titers from small volumes of cell culture supernatants using multivariate analysis and partial least squares regression of UV-Vis spectra

• Main Authors: Salinthip Jarusintanakorn, Chutima Phechkrajang, Putthiporn Khongkaew, Enrico Mastrobattista, Montarop Yamabhai
• Other Authors: Utrechts Instituut voor Farmaceutische Wetenschappen
• Format: Article
• Published: 2022
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/76050
• Institution: Mahidol University

Antibody titer and viable cell density (VCD) are two important parameters that need to be closely monitored during the process of cell line development and manufacturing of therapeutic antibodies. Typically, determination of each parameter requires 10–100 μL of supernatant sample, which is not suita...