High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology
Amodiaquine is a drug used for treatment of malaria and is often used in combination with artesunate in areas where malaria parasites are still susceptible to amodiaquine. Liquid chromatography tandem-mass spectrometry was used to quantify amodiaquine and its active metabolite, desethylamodiaquine,...
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th-mahidol.760852022-08-04T15:22:53Z High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology Karnrawee Kaewkhao Joel Tarning Daniel Blessborn Faculty of Tropical Medicine, Mahidol University Nuffield Department of Medicine Biochemistry, Genetics and Molecular Biology Chemistry Amodiaquine is a drug used for treatment of malaria and is often used in combination with artesunate in areas where malaria parasites are still susceptible to amodiaquine. Liquid chromatography tandem-mass spectrometry was used to quantify amodiaquine and its active metabolite, desethylamodiaquine, in plasma samples. A low sample volume of 100 µl, and high-throughput extraction technique using a supported liquid extraction (SLE+) technique on an automated liquid handler platform for faster sample processing are some of the advantages of this method. Separation of amodiaquine from desethylamodiaquine was achieved using a reversed phase Zorbax SB-CN 50 mm × 4.6 mm, I.D. 3.5 µm column with acetonitrile and 20 mM ammonium formate with 1% formic acid pH ~ 2.6 (15–85, v/v) as mobile phase. The absolute recoveries of amodiaquine and desethylamodiaquine were 66% to 76%, and their isotope label internal standard were in the range of 73% to 85%. Validation results of the developed method demonstrated intra-batch and inter-batch precisions within the acceptance criteria range of ± 15.0%. There were no matrix or carry-over effects observed. The lower limit of quantification was 1.08 ng/ml for amodiaquine and 1.41 ng/ml for desethylamodiaquine. The method showed robust and accurate performance with high sensitivity. Thus, the validated method was successfully implemented and applied in the evaluation of a clinical trial where participants received artemether–lumefantrine plus amodiaquine twice daily for three days (amodiaquine dose of 10 mg base/kg/day). 2022-08-04T08:06:59Z 2022-08-04T08:06:59Z 2021-08-01 Article Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol.1179, (2021) 10.1016/j.jchromb.2021.122887 1873376X 15700232 2-s2.0-85111800757 https://repository.li.mahidol.ac.th/handle/123456789/76085 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85111800757&origin=inward |
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Biochemistry, Genetics and Molecular Biology Chemistry Karnrawee Kaewkhao Joel Tarning Daniel Blessborn High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
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Amodiaquine is a drug used for treatment of malaria and is often used in combination with artesunate in areas where malaria parasites are still susceptible to amodiaquine. Liquid chromatography tandem-mass spectrometry was used to quantify amodiaquine and its active metabolite, desethylamodiaquine, in plasma samples. A low sample volume of 100 µl, and high-throughput extraction technique using a supported liquid extraction (SLE+) technique on an automated liquid handler platform for faster sample processing are some of the advantages of this method. Separation of amodiaquine from desethylamodiaquine was achieved using a reversed phase Zorbax SB-CN 50 mm × 4.6 mm, I.D. 3.5 µm column with acetonitrile and 20 mM ammonium formate with 1% formic acid pH ~ 2.6 (15–85, v/v) as mobile phase. The absolute recoveries of amodiaquine and desethylamodiaquine were 66% to 76%, and their isotope label internal standard were in the range of 73% to 85%. Validation results of the developed method demonstrated intra-batch and inter-batch precisions within the acceptance criteria range of ± 15.0%. There were no matrix or carry-over effects observed. The lower limit of quantification was 1.08 ng/ml for amodiaquine and 1.41 ng/ml for desethylamodiaquine. The method showed robust and accurate performance with high sensitivity. Thus, the validated method was successfully implemented and applied in the evaluation of a clinical trial where participants received artemether–lumefantrine plus amodiaquine twice daily for three days (amodiaquine dose of 10 mg base/kg/day). |
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Faculty of Tropical Medicine, Mahidol University |
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Faculty of Tropical Medicine, Mahidol University Karnrawee Kaewkhao Joel Tarning Daniel Blessborn |
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Article |
author |
Karnrawee Kaewkhao Joel Tarning Daniel Blessborn |
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Karnrawee Kaewkhao |
title |
High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
title_short |
High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
title_full |
High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
title_fullStr |
High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
title_full_unstemmed |
High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
title_sort |
high-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology |
publishDate |
2022 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/76085 |
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1763488902890389504 |