Improved yeast-screening assay for phytoestrogens

• Main Authors: Natthida Sophon, Choladda Phiwpesh, Sieng Darith, Wichai Cherdshewasart, Chuenchit Boonchird
• Other Authors: Chulalongkorn University
• Format: Article
• Published: 2022
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/76163
• Institution: Mahidol University

A yeast estrogen screening (YES) assay was improved to increase sensitivity for detection of phytoestrogens. New yeast strains minus one or the other of transporters Pdr5 or Snq2 and harboring yEGFP as a reporter gene were developed. The new strains showed 2-100-fold improvement in sensitivity for d...