The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells

Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of Andrographis paniculata, and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the pre...

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Main Authors: Thitianan Kulsirirat, Sittisak Honsawek, Mariko Takeda-Morishita, Nuttanan Sinchaipanid, Wanvisa Udomsinprasert, Jiraporn Leanpolchareanchai, Korbtham Sathirakul
Other Authors: Chulalongkorn University
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Published: 2022
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/76226
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spelling th-mahidol.762262022-08-04T18:22:33Z The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells Thitianan Kulsirirat Sittisak Honsawek Mariko Takeda-Morishita Nuttanan Sinchaipanid Wanvisa Udomsinprasert Jiraporn Leanpolchareanchai Korbtham Sathirakul Chulalongkorn University Mahidol University Kobe Gakuin University Biochemistry, Genetics and Molecular Biology Chemistry Pharmacology, Toxicology and Pharmaceutics Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of Andrographis paniculata, and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the present study, our goal was to evaluate the innovative effects of andrographolide on viability and Tri-lineage differentiation of human mesenchymal stem cells from suprapatellar fat pad tissues. The results revealed that andrographolide had no cytotoxic effects when the concentration was less than 12.5 µM. Interestingly, andrographolide had significantly enhanced, dose dependent, osteogenesis and chondrogenesis as evidenced by a significantly intensified stain for Alizarin Red S, Toluidine Blue and Alcian Blue. Moreover, andrographolide can upregulate the expression of genes related to osteogenic and chondrogenic differentiation, including Runx2, OPN, Sox9, and Aggrecan in mesenchymal stem cells from human suprapatellar fat pad tissues. In contrast, andrographolide suppressed adipogenic differentiation as evidenced by significantly diminished Oil Red O staining and expression levels for adipogenic-specific genes for PPAR-γ2 and LPL. These findings confirm that andrographolide can specifically enhance osteogenesis and chondrogenesis of mesenchymal stem cells from human suprapatellar fat pad tissues. It has potential as a therapeutic agent derived from natural sources for regenerative medicine. 2022-08-04T08:10:36Z 2022-08-04T08:10:36Z 2021-04-01 Article Molecules. Vol.26, No.7 (2021) 10.3390/molecules26071831 14203049 2-s2.0-85103919943 https://repository.li.mahidol.ac.th/handle/123456789/76226 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85103919943&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
Thitianan Kulsirirat
Sittisak Honsawek
Mariko Takeda-Morishita
Nuttanan Sinchaipanid
Wanvisa Udomsinprasert
Jiraporn Leanpolchareanchai
Korbtham Sathirakul
The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
description Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of Andrographis paniculata, and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the present study, our goal was to evaluate the innovative effects of andrographolide on viability and Tri-lineage differentiation of human mesenchymal stem cells from suprapatellar fat pad tissues. The results revealed that andrographolide had no cytotoxic effects when the concentration was less than 12.5 µM. Interestingly, andrographolide had significantly enhanced, dose dependent, osteogenesis and chondrogenesis as evidenced by a significantly intensified stain for Alizarin Red S, Toluidine Blue and Alcian Blue. Moreover, andrographolide can upregulate the expression of genes related to osteogenic and chondrogenic differentiation, including Runx2, OPN, Sox9, and Aggrecan in mesenchymal stem cells from human suprapatellar fat pad tissues. In contrast, andrographolide suppressed adipogenic differentiation as evidenced by significantly diminished Oil Red O staining and expression levels for adipogenic-specific genes for PPAR-γ2 and LPL. These findings confirm that andrographolide can specifically enhance osteogenesis and chondrogenesis of mesenchymal stem cells from human suprapatellar fat pad tissues. It has potential as a therapeutic agent derived from natural sources for regenerative medicine.
author2 Chulalongkorn University
author_facet Chulalongkorn University
Thitianan Kulsirirat
Sittisak Honsawek
Mariko Takeda-Morishita
Nuttanan Sinchaipanid
Wanvisa Udomsinprasert
Jiraporn Leanpolchareanchai
Korbtham Sathirakul
format Article
author Thitianan Kulsirirat
Sittisak Honsawek
Mariko Takeda-Morishita
Nuttanan Sinchaipanid
Wanvisa Udomsinprasert
Jiraporn Leanpolchareanchai
Korbtham Sathirakul
author_sort Thitianan Kulsirirat
title The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
title_short The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
title_full The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
title_fullStr The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
title_full_unstemmed The effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
title_sort effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells
publishDate 2022
url https://repository.li.mahidol.ac.th/handle/123456789/76226
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