BRET-based assay to specifically monitor β<inf>2</inf>AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation

• Main Authors: Warisara Parichatikanond, Ei Thet Htar Kyaw, Corina T. Madreiter-Sokolowski, Supachoke Mangmool
• Other Authors: Mahidol University
• Format: Book Chapter
• Published: 2022
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/76361

The β-adrenergic receptors (βARs) are members of G protein-coupled receptor (GPCR) family and have been one of the most important GPCRs for studying receptor endocytosis and signaling pathway. Agonist binding of βARs leads to an activation of G proteins and their canonical effectors. In a parallel w...