A Comparison between Sonication and Homogenization Methods for Extraction of Mosquito Allergens
Objective: To compare sonication and homogenization methods for preparation of mosquito allergen extracts. Materials and Methods: Mosquito allergens were extracted from Culex quinquefasciatus using two different methods, sonication and homogenization. Protein concentrations, protein profiles, and e...
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| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Original Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/79604 |
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| Institution: | Mahidol University |
| Language: | English |
| Summary: | Objective: To compare sonication and homogenization methods for preparation of mosquito allergen extracts.
Materials and Methods: Mosquito allergens were extracted from Culex quinquefasciatus using two different methods, sonication and homogenization. Protein concentrations, protein profiles, and endotoxin levels in the extracts prepared by the two methods were determined.
Results: The protein concentrations measured by Bradford (results in mg/mL) and Kjeldahl (results in Protein Nitrogen Units, PNU) assays in the extracts prepared by the sonication method were 0.92 gif.latex?\pm 0.08 mg/mL and 74700 gif.latex?\pm 4934 PNU, respectively. These results were significantly higher than those in the extracts prepared by the homogenization method (0.64 gif.latex?\pm 0.01 mg/mL and 41330 gif.latex?\pm 8231 PNU). The protein fingerprint showed more protein in the extracts prepared by sonication than in those prepared by homogenization. The endotoxin levels in the extracts from both methods were clinically acceptable, and there was no bacterial contamination.
Conclusion: Sonication provides mosquito allergen extracts with higher quantities of proteins and better protein composition than homogenization. |
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