The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm
This study aimed to determine the effect of resveratrol and its optimal concentration on the quality of frozen-thawed (FT) boar sperm. Semen ejaculates were obtained from 13 Duroc boars aged between 1.5 and 3 years. The sperm sample was separated into 7 groups based on the concentrations of resverat...
Saved in:
Main Author: | |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2023
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/90306 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.90306 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.903062023-10-05T01:02:57Z The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm Kaeoket K. Mahidol University Veterinary This study aimed to determine the effect of resveratrol and its optimal concentration on the quality of frozen-thawed (FT) boar sperm. Semen ejaculates were obtained from 13 Duroc boars aged between 1.5 and 3 years. The sperm sample was separated into 7 groups based on the concentrations of resveratrol in the freezing extender, which were 0 (control), 25, 50, 75, 100, 125, and 250 µM, respectively. The sperm was frozen using liquid nitrogen vapor and thawed at 50 °C for 12 s. After thawing, total motility, progressive motility, viability, intact acrosomes, mitochondrial membrane potential and level of MDA were assessed. The supplementation of 50–100 µM resveratrol improved the sperm motility and viability of FT sperm in comparison to the control group (p < 0.05). Furthermore, the 50 µM resveratrol group was significantly more protective than the control group in terms of intact acrosome, mitochondrial membrane potential, and level of MDA (p < 0.05). Nonetheless, the detrimental effect of resveratrol was found at a concentration of 250 µM. In conclusion, the addition of 50–100 µM resveratrol to a freezing extender is the optimal concentration for enhancing the quality of cryopreserved boar sperm. 2023-10-04T18:02:57Z 2023-10-04T18:02:57Z 2023-09-01 Article Animals Vol.13 No.18 (2023) 10.3390/ani13182829 20762615 2-s2.0-85172286256 https://repository.li.mahidol.ac.th/handle/123456789/90306 SCOPUS |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Veterinary |
spellingShingle |
Veterinary Kaeoket K. The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm |
description |
This study aimed to determine the effect of resveratrol and its optimal concentration on the quality of frozen-thawed (FT) boar sperm. Semen ejaculates were obtained from 13 Duroc boars aged between 1.5 and 3 years. The sperm sample was separated into 7 groups based on the concentrations of resveratrol in the freezing extender, which were 0 (control), 25, 50, 75, 100, 125, and 250 µM, respectively. The sperm was frozen using liquid nitrogen vapor and thawed at 50 °C for 12 s. After thawing, total motility, progressive motility, viability, intact acrosomes, mitochondrial membrane potential and level of MDA were assessed. The supplementation of 50–100 µM resveratrol improved the sperm motility and viability of FT sperm in comparison to the control group (p < 0.05). Furthermore, the 50 µM resveratrol group was significantly more protective than the control group in terms of intact acrosome, mitochondrial membrane potential, and level of MDA (p < 0.05). Nonetheless, the detrimental effect of resveratrol was found at a concentration of 250 µM. In conclusion, the addition of 50–100 µM resveratrol to a freezing extender is the optimal concentration for enhancing the quality of cryopreserved boar sperm. |
author2 |
Mahidol University |
author_facet |
Mahidol University Kaeoket K. |
format |
Article |
author |
Kaeoket K. |
author_sort |
Kaeoket K. |
title |
The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm |
title_short |
The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm |
title_full |
The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm |
title_fullStr |
The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm |
title_full_unstemmed |
The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm |
title_sort |
beneficial effect of resveratrol on the quality of frozen-thawed boar sperm |
publishDate |
2023 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/90306 |
_version_ |
1781797356897828864 |