Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration
Doctor of Philosophy (Pharmaceutical Sciences), 2023
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Prince of Songkla University
2023
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th-psu.2016-192592023-12-20T09:10:33Z Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration Bintang, Muhammad Ali Khumaini Mudhar Teerapol Srichana Faculty of Pharmaceutical Sciences (Pharmaceutical Technology) คณะเภสัชศาสตร์ ภาควิชาเทคโนโลยีเภสัชกรรม Colistin Sodium deoxycholate sulfate Micelles Nephrotoxicity Neurotoxicity Molecular docking Histopathology. Doctor of Philosophy (Pharmaceutical Sciences), 2023 Colistin is still used in multidrug-resistant gram-negative bacteria (MDR-GNB) therapy but the nephrotoxicity and neurotoxicity still become a major setback for its clinical use. Sodium deoxycholate sulfate (SDCS) carrier is used in micelle formulation with colistin to mitigate the known toxicity of colistin. The surface property of colistin and SDCS were evaluated before formulation with the lyophilisation technique. Several physicochemical parameters were evaluated like particle size, zeta potential, morphology, encapsulation efficiency (EE) and release profile. The chemical interactions were analysed using FTIR, NMR and molecular docking. The cytotoxicity of formulations was tested with different kidney cell lines. The in vivo toxicity was carried out in male mice C57BL/6 with colistin and colistin formulation for 7 consecutive days. The physiological changes were observed and measured after treatment. The serum biomarkers were measured including blood urea nitrogen (BUN), creatinine (Cr), superoxide dismutase (SOD), and catalase (CAT). Histopathological alterations in mice organs were analysed. The hydrodynamic diameters of the formulation were in the range of 140 to 170 nm with a spherical shape and negative zeta potential between −35.3 and −22.8 mV. The EE of formulations were between 70 and 76.4% with slower release measured compared to colistin. Molecular interactions were determined from FTIR and NMR spectra. Molecular docking simulation showed that multiple hydrogen bonds were present between the hydrophilic ring of colistin and SDCS. The colistin:SDCS formulations improved the thermal sensitivity of the mice compared to the control group. The BUN and Cr results showed no significant kidney dysfunction; however, the oxidative stress biomarkers decreased in the colistin with a lesser decrease in colistin-SDCS treated mice. Several histological alterations were observed in the kidney, liver, spleen, and sciatic nerve tissues following colistin treatment, whereas less evidence of toxicity with colistin-SDCS. The overall results indicated that micelle formulations with SDCS showed safer for kidney and nerve cells while maintaining the antibacterial activity of colistin. This study revealed the potential for colistin development with SDCS for safer clinical use against MDR-GNB. 2023-12-20T09:09:33Z 2023-12-20T09:09:33Z 2023 Thesis http://kb.psu.ac.th/psukb/handle/2016/19259 en Attribution-NonCommercial-NoDerivs 3.0 Thailand http://creativecommons.org/licenses/by-nc-nd/3.0/th/ application/pdf Prince of Songkla University |
| institution |
Prince of Songkhla University |
| building |
Khunying Long Athakravi Sunthorn Learning Resources Center |
| continent |
Asia |
| country |
Thailand Thailand |
| content_provider |
Khunying Long Athakravi Sunthorn Learning Resources Center |
| collection |
PSU Knowledge Bank |
| language |
English |
| topic |
Colistin Sodium deoxycholate sulfate Micelles Nephrotoxicity Neurotoxicity Molecular docking Histopathology. |
| spellingShingle |
Colistin Sodium deoxycholate sulfate Micelles Nephrotoxicity Neurotoxicity Molecular docking Histopathology. Bintang, Muhammad Ali Khumaini Mudhar Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| description |
Doctor of Philosophy (Pharmaceutical Sciences), 2023 |
| author2 |
Teerapol Srichana |
| author_facet |
Teerapol Srichana Bintang, Muhammad Ali Khumaini Mudhar |
| format |
Theses and Dissertations |
| author |
Bintang, Muhammad Ali Khumaini Mudhar |
| author_sort |
Bintang, Muhammad Ali Khumaini Mudhar |
| title |
Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| title_short |
Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| title_full |
Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| title_fullStr |
Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| title_full_unstemmed |
Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| title_sort |
formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration |
| publisher |
Prince of Songkla University |
| publishDate |
2023 |
| url |
http://kb.psu.ac.th/psukb/handle/2016/19259 |
| _version_ |
1787137887601426432 |