Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan

Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan

a-Amylase, 1,4-a-D-glucan glucanohydrolase (EC 3.2.1.1) is a biocatalyze which hydrolyses a-1,4 glycosidic linkages of starch to produce oligosaccharide and dextrin. a-Amylases from microorganism have been studied but only a few marine organism identified have an a–amylases activities. Kakaba...

Full description

Saved in:
Bibliographic Details
Main Author: SETIYABUDI (NIM 10505090); Pembimbing: Dr. Dessy Natalia, LULU
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/13618
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:13618
spelling id-itb.:136182017-09-27T11:42:37ZScreening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan SETIYABUDI (NIM 10505090); Pembimbing: Dr. Dessy Natalia, LULU Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/13618 a-Amylase, 1,4-a-D-glucan glucanohydrolase (EC 3.2.1.1) is a biocatalyze which hydrolyses a-1,4 glycosidic linkages of starch to produce oligosaccharide and dextrin. a-Amylases from microorganism have been studied but only a few marine organism identified have an a–amylases activities. Kakaban lake is a landlocked marine lake. The purpose of this research was to screen, isolate and characterize of potato degrading a–amylase from Kakaban lake isolates, East Kalimantan. Screening of a–amylase activity from 40 Kakaban Lake isolates by red amylopectine gave 6 isolates showing starch degrading activity. Morphology and physiology analysis of 16S rDNA gene show that isolates KBU 1-6L and KBU 2-FL are Bacillus epiphytus and Pseudoalteromonas sp., respectively. Zimogram analysis also showed that they secrete a-amylases. The activity of isolate KBU 1-6L a-amilase from saturated ammonium sulphate 0-80% fraction at 50 oC, pH 7.0 was 169. 17 U/mL. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
description a-Amylase, 1,4-a-D-glucan glucanohydrolase (EC 3.2.1.1) is a biocatalyze which hydrolyses a-1,4 glycosidic linkages of starch to produce oligosaccharide and dextrin. a-Amylases from microorganism have been studied but only a few marine organism identified have an a–amylases activities. Kakaban lake is a landlocked marine lake. The purpose of this research was to screen, isolate and characterize of potato degrading a–amylase from Kakaban lake isolates, East Kalimantan. Screening of a–amylase activity from 40 Kakaban Lake isolates by red amylopectine gave 6 isolates showing starch degrading activity. Morphology and physiology analysis of 16S rDNA gene show that isolates KBU 1-6L and KBU 2-FL are Bacillus epiphytus and Pseudoalteromonas sp., respectively. Zimogram analysis also showed that they secrete a-amylases. The activity of isolate KBU 1-6L a-amilase from saturated ammonium sulphate 0-80% fraction at 50 oC, pH 7.0 was 169. 17 U/mL.
format Final Project
author SETIYABUDI (NIM 10505090); Pembimbing: Dr. Dessy Natalia, LULU
spellingShingle SETIYABUDI (NIM 10505090); Pembimbing: Dr. Dessy Natalia, LULU
Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan
author_facet SETIYABUDI (NIM 10505090); Pembimbing: Dr. Dessy Natalia, LULU
author_sort SETIYABUDI (NIM 10505090); Pembimbing: Dr. Dessy Natalia, LULU
title Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan
title_short Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan
title_full Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan
title_fullStr Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan
title_full_unstemmed Screening, Isolation and Characterization of Potato Starch Degrading a-Amylase from Kakaban Lake Isolates, East Kalimantan
title_sort screening, isolation and characterization of potato starch degrading a-amylase from kakaban lake isolates, east kalimantan
url https://digilib.itb.ac.id/gdl/view/13618
_version_ 1820736240054435840

Similar Items