Increasing Protein Hydrolyzate Yield from The Residue of Lutein and Zeaxanthin Extract of Sweet Potatoes (Ipomoea batatas L.) Leaves Varieties Cilembu Through Pre-Treatment Residue with Aspergillus niger Mushrooms

• Main Author: Ayu Prawitasari, Diah
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40025
• Institution: Institut Teknologi Bandung

Sweet potato (Ipomoea batatas L.) is a root tuber plant that has contain calories, nutrients, and minerals for human needs. The productivity of sweet potato leaves is 5-6 tons/ha/ year. Sweet potato leaves contain secondary metabolites which are very potential to be utilized, ?-carotene. Extraction process of these compounds leaves a huge residue. One option for utilization of the side product from the extraction of ?-carotene was as a subtrate for hydrolysis to obtain hydrolysate protein. Sweet potato leaf pulp was treated first with fermentation with Aspergillus niger to enrich the protein. Protein contain of fermented Aspergillus niger in 4, 7, 10, and 14 days is 15.49% (dw); 17.91% (dw); 20.2% (dw); and 19.13% (dw). Leaf pulp with the highest protein yield is at day 10 which is used as main subtrate for protein hydrolyzates. The oil and its moisture content is removed from the pulp and then grind into the flour with size of 120 mesh. The amount of bromelain enzyme used is twice the amount protein content of sweet potato leaf pulp. From the results of hydrolysis, the liquid volume of hydrolyzate treatment is 61 ml and the control is 43 ml. Concentration of dissolved proteins and amino acids was tested using the BCA (Bicinchonic Acid Assay) method. Dissolved protein and amino acid content of the hydrolyzated treatment is 0.046 g / ml while the control is 0.028 g / ml. Yield of dissolved protein and amino acids content in the hydrolyzated pre-treatment is 0.6 ????/???? ???????????? ???????????????????????????????? while the control is 0.35 ????/???? ???????????? ???????????????????????????????? . However that yield is still contain the enzyme bromelain content. Yield of pure dissolved proteins and pure amino acids of treated hydrolysate without the enzyme is 0.19 ????/???? ???????????? ???????????????????????????????? and control is 0.1????/???? ???????????? ???????????????????????????????? .