CONSORTIUM OF LACTIC ACID BACTERIA FOR BIOPRESERVATIVE DEVELOPMENT
Lactic acid bacteria (LAB) is commonly used in food processing and known to produce secondary metabolite showing antimicrobial activity and hence can be developed as biopreservative. The development and utilization of LAB can be conducted by single culture microbe as well as microbial consortium....
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40133 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Lactic acid bacteria (LAB) is commonly used in food processing and known to
produce secondary metabolite showing antimicrobial activity and hence can be
developed as biopreservative. The development and utilization of LAB can be
conducted by single culture microbe as well as microbial consortium. The aim of
this research was to study the activity of bacterial consortium consist of
Bifidobacterium, Lactobacillus plantarum 14-BL and Pediococcus acidilactici F11 in producing bacteriosine as antimicrobe using edible growing media made of
sweet potato. Consortium ability of the three LAB was conducted by synergetic test.
Optimum mixing time of the three LAB and optimum fermentation time to produce
bacteriocine-rich supernatant was determined by means of growth curve plotted
between turbidity and bacterial cell counting versus time. Bacteriocine-containing
supernatant was prepared by culture centrifugation and subsequent pH adjustment
in the range of 6 to 7. Antimicrobial activity of the supernatant was tested on E.
coli, S. aureus, A. flavus, and C. albicans using agar diffusion method,
determination of total plate count (TPC), and determination of minimum inhibition
concentration (MIC). Optimum fermentation was achieved at 20 to 24 hours
incubation time. Bacteriocine-containing supernatant showed antimicrobial
activity on E. coli only, while in the case of TPC reduced colony count of E. coli
and S. aureus was observed due to addition of this supernatant. MIC determination
results revealed that MICs of the supernatant on E. coli and S. aureus were 25 and
100% v/v, respectively. Based on overall results, it was concluded that
Bacteriocine-containing supernatant prepared from LAB consortium showed
antibacterial activity and hence has the potency to be biopreservative.
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