PENGEMBANGAN BIOPRESERVATIVE DARI LACTOBACILLUS PLANTARUM UNTUK MEMPERPANJANG DURASI SIMPAN TAHU

• Main Author: Puspamurti, Ester
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44319
• Institution: Institut Teknologi Bandung

Tofu is one of food products which needs preservative agent. The biopreservative as alternative preservative for tofu can be developed from Lactobacillus plantarum, a Gram-positive bacteria which can produces plantaricin, a bacteriocin, that has antimicrobe activity. The recommended media for the optimum growth of L. plantarum is MRS. However due to expensive price, alternative media has to be sought. Previous study revealed that sweet potato is a suitable media for Lactobacillus plantarum. The aim of this study is to determine activity of plantaricin containing supernatant for tofu preservation and to obtain optimum transformation condition of liquid supernatant into its solid powder. Plantaricin containing supernatant was obtained from the alternative media by centrifugation and filtration and after addition of suitable vehicle subsequently transformed into its solid powder form by means of spray drying method. The plantaricin containing supernatant has the density of 1.13 g/mL and pH value of 6.78, while its solid powder contained total volatile compound of 2.07%. Antimicrobe activity test using agar diffusion with paper disk on Escherichia coli ATCC 8739 showed that the inhibition zone diameter of 100, 50, 25% of plantaricin containing supernatant were 12±1.32, 9.83±0.29, 8.17±0.29 mm, respectively, while that of its solid powder equivalent to 50% plantaricin containing supernatant, was 8.75±1.01 mm. Organoleptic observation showed that tofu without treatment changed its texture and smell after two days storage at room temperature, while those of plantaricin containing supernatant and solid powder treated at the concentration level of 50% with identical storage condition, first after four and five day’s storage, respectively. Applying total plate count test, the number of colonies tofu immersion water without treatment was >500 cfu/mL, while those of plantaricin containing supernatant and solid powder treated were 172, 74, 405, and 77 cfu/mL, respectively.