UJI IHIBISI MINYAK BIJI KENARI (CANARIUM INDICUM L.) TERHADAP AKTIVITAS ENZIM TIROSINASE

• Main Author: Dinny Hasan, Shafira
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44334
• Institution: Institut Teknologi Bandung

Inhibition of tyrosinase activity, the enzyme that play a catalytic role in forming skin pigment, is one effort to overcome hyperpigmentation. Exploration of the use of natural ingredients that have inhibitory activity on tyrosinase continues to evolve to date, related to consumers need for good and safe cosmeceuticals products. The purpose of this study was to evaluate inhibition of tyrosinase activity in canary nut oil. The test sample consists of a saturated and unsaturated canary nut oil. Tests were conducted in vitro through a review of the decrease of tyrosinase activity to L-DOPA as substrate. Each sample was dissolved in 25% DMSO with some variation of sample concentration. The test was performed by adding L-DOPA and tyrosinase which were dissolved in phosphate buffer pH 6.5. The concentration of tyrosinase and L-DOPA solution was optimized by carrying incubation at room temperature. The inhibitory activity of the sample was observed of decreasing the amount of dopachrome as reaction product i.e. decreasing the optical absorbance compared to the treatment without adding the sample, at a wavelength of 475 nm. Kojic acid was used as positive control. The test results showed that canary nut oil has inhibitory enzymatic activity towards tyrosinase which was relatively much lower than kojic acid. Addition of unsaturated canary nut oil at a concentration of 10 mg/mL resulted a decreased tyrosinase enzymatic activity to 51%, while the same concentration of saturated canary nut oil decreased tyrosinase activity to 73.3%.