THE EFFECT OF DRYING ON THE AMOUNT OF CHLOROGENIC ACID IN COFFEA ARABICA (L.) AND COFFEA CANEPHORA (L.) BEANS

• Main Author: Qistina Sulaiman, Nur
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45071
• Institution: Institut Teknologi Bandung

Determination of the plant was done in Herbarium Bandungense, The School of Life Science and Technology, Bandung Institute of Technology. The result of determination confirmed that the plant used was Coffea arabica (L.) and Coffea canephora (L.) from Rubiaceae family. The crude drug was characterized for its water content for Coffea arabica (L.) were 5.67% v/w, total ash content 3.34%, acid insoluble ash content 0.65%, water soluble extractable matter 24.63%, ethanol soluble extractable matter 16.85% and loss of drying 7.88%. Characterization for Coffea canephora (L.) for water content were 5.3% v/w, total ash content 3.46%, acid insoluble ash content 0.79%, water soluble extractable matter 56.40%, ethanol soluble extractable matter 42.67% and loss of drying 6.37%. Phytochemical screening results of coffee beans for both species showed the presence of flavonoid, alkaloid, phenol, catechuic tannin and steroid/triterpenoid. The coffee beans from Coffea arabica (L.) and Coffea canephora (L.) species were drying at two conditions which were in air circulated oven at different temperature and under the sun. The drying temperature was observed at 40 60 , 80 and 100 . The crude drug was macerated by using methanol as solvents for 24 hours and analyzed the quantities of chlorogenic acid by using TLC Scanner 3. The samples together with standard was spot onto TLC plate by using linomat 5 and developed by using ethyl acetate: formic acid: water (8:1:1) as mobile phase. The sample of both species Coffea arabica (L.) and Coffea canephora (L.) was measured their chlorogenic acid content by using TLC Scanner 3 (CAMAG) at 329 nm. The result from analysis showed that chlorogenic acid contents in both species Coffea arabica (L.) and Coffea canephora (L.) was highest when dry at 40 compared when drying at 60 , , 100 and under the sun. This is due to that activity of peroxidase was optimum at 60 . At above 60 , the activity of peroxidase may decrease due to the degradation of the enzyme. The data showed that for both species the highest amount of chlorogenic acid was in immatured beans followed by semimatured and matured. Furthermore, the Coffea canephora (L.) species showed higher amount of chlorogenic acid compared to Coffea arabica (L.).