POTENSI BAKTERI PROBIOTIK LACTOBACILLUS PLANTARUM PADA MEDIUM SUSU KAMBING SEBAGAI BAHAN BAKU KOSMETIK

• Main Author: Melati Widianwari, Anisa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/50656
• Institution: Institut Teknologi Bandung

Microflora is an important component of the skin. Moisturizing cream is one of the cosmetic products that many people use in common. However, moisturizing creams can damage the microflora composition of the skin. The addition of a probiotic bacteria to facial moisturizing creams is an innovation that is considered useful in balancing the number of microflora. Therefore, it is considered as an efficient solution to deal with several skin problems. Lactobacillus plantarum is a probiotic bacteria which is commonly used as raw material for moisturizing cream. Preliminary research was conducted to determine the ability of L. plantarum to inhibit the growth of indicator bacteria, Staphylococcus aureus and Pseudomonas aeruginosa with qualitative Cultural Compatibility Test and quantitative testing through: (1) Kirby-Bauer method between L. plantarum (108 CFU / mL) and variations concentration of antibiotic erythromycin against indicator bacteria; and (2) Microdilution broth to determine the Minimum Inhibitory Concentration (MIC) value, which is the lowest concentration of L. plantarum cell numbers and variations in the concentration of erythromycin antibiotics against indicator bacteria using spectrophotometry. Furthermore, L. plantarum was cultured in a goat milk medium with inoculum of 10% (v/v), the initial pH of the medium was 6.72 and incubated at 37o C for 24 hours. The Total Plate Count analysis, pH changes, and total acid production were condecuted in this study. Based on the results of quantitative and qualitative tests, it shows that L. plantarum has the ability of inhibiting the growth of indicator bacteria which produces a zone of inhibition in S. aureus (10 ± 0.44mm) and P. aeruginosa (10 ± 0.33mm) which is equivalent to the ability of erythromycin concentration 64 ?g / mL. From the MIC results, it is known that the minimum cell count of L. plantarum in inhibiting S. aureus and P. aeruginosa is 108 CFU / ml which is equivalent to the ability of erythromycin antibiotics to inhibit S. aureus and P. aeruginosa each of 4 ?g / mL and 40?g / mL. The results of L. plantarum growth in goat milk medium had a cell growth rate of 0.22 cells / hour, a change in pH from 6.72 to 5.18 with a decrease in pH of 0.06 / hour and acid production began at 6 hours with the highest production. total acid 2.25 x 10-3 ppm and the rate of formation of total organic acids 3.64 ppm / hour. Inhibition test and analysis of the resulting metabolites have not been carried out.