PENGEMBANGAN METODE ANALISIS KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENENTUAN KADAR ALITAM, KALIUM ASESULFAM, DAN ASPARTAM

• Main Author: Mulyani, Reni
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/5320
• Institution: Institut Teknologi Bandung

<b>Abstract:</b> <p align=\"justify\"> <br /> <br /> <br /> Analytical method of alitame, potasium acesulfame, and aspartame in syrup, orange juice, milk and biscuit using high performance liquid chromatography with a Partisil ODS C-18 column as stasionary phase, a mixture of methanol and phosphate buffer pH 5.3 (32:68) as mobile phase, a flow rate of 1 mL/minute and detected at 214 nm had been studied. Result showed that the recovery of alitame, potasium acesulfame and aspartame in syrup were ((99.76±0.22), (99.71±0.42) and (99.36±0.66) % respectively, in orange juice (99.11±0.79), (99.27±0.29) and (99.44±0.57) % respectively, in milk (98.44±0.59), (98.48±0.74) and (97.41±0.95) % respectively, and in biscuits (99.64±0.23), (94.66±0.21) and (94.46±0.26) % respectively. The method gave significant accuracy and precission, so it can be used for determination of these sweetening agents.<p align=\"justify\">