PEMANFAATAN KOKULTUR ASPERGILLUS ORYZAE, SACCHAROMYCES CEREVISIAE DAN RHIZOPUS OLIGOSPORUS PADA MEDIA KULIT BUAH PADI, KULIT UMBI UBI JALAR DAN KULIT UMBI UBI KAYU UNTUK PENINGKATAN KADAR PROTEIN TOTAL DALAM PAKAN TERNAK

• Main Author: Husni , Ahmad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/5677
• Institution: Institut Teknologi Bandung

The availability of rice bran, sweet potato and cassava peels in Indonesia is very large so that it is potential to be reprocessed into animal feed product with additional value. This research aimed to study the utilization of co-culture of Aspergillus oryzae, Saccharomyces cerevisiae and Rhizopus oligosporus using solid substrate fermentation technique to produce high-protein animal feed. This experiment started with optimization of several fermentation parameters, continued with the preparation of tested samples and the determination of total protein and total sugar content in the final product. The determination of total protein content was carried out by Kjeldahl method and the determination of total sugar content was carried out by Soxhlet modified Fehling method. The result of fermentation process for seven days showed that the rice bran is the best media to grow the fungal inoculum compared to the other media compositions under examination in this research. The rice bran inoculated with Aspergillus oryzae and Saccharomyces cerevisiae yielded the highest total protein content, as much as 47.78 +/- 0.96 % (w/w) while the one inoculated only with Aspergillus oryzae yielded 39.19 +/- 0.9 % (w/w). On the contrary, the rice bran inoculated only with Saccharomyces cerevisiae experienced a decrease in total protein content up to 7.69 +/- 0.65 % (w/w). Rhizopus oligosporus was not used anymore since it didnot show any growth in all media composition under examination.