PRELIMINARY STUDY OF LABELLING ESSENSIAL FATTY ACID AND FACTOR-2 (6,7,4-TRIHIDROXYISOFLAVONE) FROM RHIZOPUS OLIGOSPORUS-FERMENTED OIL

• Main Author: Tresnaeni (NIM 105 01 005), Arni
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/5786
• Institution: Institut Teknologi Bandung

ABSTRACT: <br /> <br /> The quality of Virgin Coconut Oil (VCO) can be improved if it contains essential fatty acid and factor-2 (6,7,4-trihydroxyisoflavone). This research aimed to trace and identify fatty acid and factor-2 from labelled coconut milk and labelled soy milk. It also aimed to study the relationship between production of essential fatty acid and factor-2. In this research, VCO was made at composition of coconut milk:soy milk at (7:3) fermented by R. oligosporus in room temperature, agitated at 150-rpm, and incubated for 3 days. Metabolits tracing was carried out using a deuterium labelling by 55% (v/v) either to coconut or soybean tissues. Deuteriums penetration to coconut and soybean were conducted by vacuum infiltration for 15 minutes to 8 hours to the respective tissues prior to the fermentation process. Factor-2 purification was carried out using TLC (C-18 Rp plate, methanol:0,2% HAc (8:2), multiple development) and identified using TLC scanner (CAMAG, lambda= 254 nm). Identification of fermentation oil and deuterium-incorporated factor-2 was conducted using GC-MS. Results from labelled coconut milk:soy milk used in this research, showed that essential fatty acid (linoleic acid) was produced along with the production of factor-2, while in coconut milk:labelled soy milk, the content of stearic acid increased along with the production of factor-2. Labelled factor-2 was not obtained in this research, on the contrary, benzene and labelled compound, detected as 1,1,1-tri-deuterium ethane, were identified in labelled coconut milk:soy milk variation. It was suspected both compound was derived from labelled factor-2 and fragmented to benzene ring and such labelled compound due to high temperature involved in the measurement using GC-MS. Identification of factor-2 isolated from coconut milk: labelled soymilk, showed unlabelled factor-2. However identification of isolated oil fraction from the same milk mixture showed labelled stearic acid in the form of 2-deuterium-glyceryl-trioctadecanoate, which was assumed as a result of R. oligosporus lipase activity. Results obtained showed that labelling of factor-2 and fermented oil have been performed in this research. Besides, it was shown that coconut as a non-legumen plants can produce factor-2 with the support of R. oligosporus enzyme activity. It was also found that there was a relationship between the production of essential fatty acid and the production of factor-2.