ISOLATION AND ANTIMICROBIAL ACTIVITY DETERMINATION OF SECONDARY METABOLITES OF MARINE-DERIVED FUNGUS ASPERGILLUS FLAVUS

• Main Author: Ghivany Gozali, Essha
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/61877
• Institution: Institut Teknologi Bandung

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. According to WHO, tuberculosis is one of the top ten causes of death in the world. Tuberculosis cases are influenced by several factors, for example, antibiotic resistant. Moreover, multiple drug resistant cases have occurred in tuberculosis where patients with multiple drug resistant cannot be treated with at least two types of strong antibiotics, such as rifampicin and isoniazid. This experiment aims to isolate new antimicrobial compound which can be applied as antibiotic for tuberculosis treatment from Aspergillus flavus isolated from Indonesian seas. Marine-derived fungus Aspergillus flavus was fermented for 30 days to produce its secondary metabolites. The mycelium and medium broth obtained from fermentation were extracted with ethyl acetate. Both extracts were tested for antimicrobial activity by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay against Mycobacterium smegmatis. Mycelium extract had better antimicrobial activity than medium broth extract with MIC value 50 ?g/mL. Mycelium extract was fractionated and tested for antimicrobial activity using the same method. Fraction 2 which had the best antimicrobial activity was separated into subfractions by High Performance Liquid Chromatography (HPLC). All collected subfractions were tested using MTT assay. Most of subfractions had MIC value 200 ?g/mL. Subfraction 2-2 had the best antimicrobial activity shown by the MIC 50 ?g/mL.