FORMULATION AND EVALUATION OF ARBUTIN SERUM IN NIOSOME AND TRANSFERSOME VESICLE SYSTEM
Arbutin is a compound with a structure similar that been used in place of hydroquinone. The production of niosomes vesicles and transfersomes is expected to increase the penetration of hydrophilic arbutin into the stratum corneum. The purpose of this study is to compare the stability and ability...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/62638 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Arbutin is a compound with a structure similar that been used in place of
hydroquinone. The production of niosomes vesicles and transfersomes is expected
to increase the penetration of hydrophilic arbutin into the stratum corneum. The
purpose of this study is to compare the stability and ability of vesicles to adsorb
arbutin, determine the safety of arbutin nanoparticles as cosmetics by in vitro test
using rabbit, and confirm their effectiveness using diffusion tests. The study was
conducted by optimating the mixing process, sonication process, and formulation.
The optimal niosomal formula was obtained from the composition of the Span20 :
Cholesterol : Arbutin (200 µmol:40 µmol:2%) and was used as a comparison of
Posphatidylcholine:Cholesterol:Arbutin with equal level in transfersome
production. Measurements of diameter and polydispersity index were performed
using a Particle Size Analyzer resulting a diameter of 290.3 ± 3.14 nm, a PI of
0.399 ± 0.01, an entrapment efficiency of 66.61 ± 1.85%, and a zeta potential of -
24.6 ± 2.29 mV for niosomes mV, and a diameter 245.13 ± 5.48 nm, PI 0.359 ±
0.02, entrapment efficiency 67.91 ± 0.99%, and zeta potential -55.27 ± 3,65 mV for
transfersome. Morphological observations with a transmission electron
microscope (TEM) showed that the vesicles of niosomes and transfersomes were
spherical with a size of <300 nm. The size of niosomes and transfersome vesicles
did not change after storage at 25 ° C and 40 ° C. Assessments of the stability of
niosomes and transfersome showed stability at pH and viscosity, but arbutin levels
decreased when stored at room temperature. Transfersomes serum is more stable
than niosomal serum and arbutin serum. Irritant tests have shown that serum
niosomes, serum transfersomes, niosomes, transfersomes, and serum bases do ot
irritate rabbit skin. The diffusion profile of niosomes serum showed the ability of
serum to release the drug is 2.3 times compared to vesicle-free serum, and 5,9 times
compared to transfersomes serum.
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