FORMULATION AND EVALUATION OF ARBUTIN SERUM IN NIOSOME AND TRANSFERSOME VESICLE SYSTEM

• Main Author: Dherrvani Terzieclara, Yuke
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62638
• Institution: Institut Teknologi Bandung

Arbutin is a compound with a structure similar that been used in place of hydroquinone. The production of niosomes vesicles and transfersomes is expected to increase the penetration of hydrophilic arbutin into the stratum corneum. The purpose of this study is to compare the stability and ability of vesicles to adsorb arbutin, determine the safety of arbutin nanoparticles as cosmetics by in vitro test using rabbit, and confirm their effectiveness using diffusion tests. The study was conducted by optimating the mixing process, sonication process, and formulation. The optimal niosomal formula was obtained from the composition of the Span20 : Cholesterol : Arbutin (200 µmol:40 µmol:2%) and was used as a comparison of Posphatidylcholine:Cholesterol:Arbutin with equal level in transfersome production. Measurements of diameter and polydispersity index were performed using a Particle Size Analyzer resulting a diameter of 290.3 ± 3.14 nm, a PI of 0.399 ± 0.01, an entrapment efficiency of 66.61 ± 1.85%, and a zeta potential of - 24.6 ± 2.29 mV for niosomes mV, and a diameter 245.13 ± 5.48 nm, PI 0.359 ± 0.02, entrapment efficiency 67.91 ± 0.99%, and zeta potential -55.27 ± 3,65 mV for transfersome. Morphological observations with a transmission electron microscope (TEM) showed that the vesicles of niosomes and transfersomes were spherical with a size of <300 nm. The size of niosomes and transfersome vesicles did not change after storage at 25 ° C and 40 ° C. Assessments of the stability of niosomes and transfersome showed stability at pH and viscosity, but arbutin levels decreased when stored at room temperature. Transfersomes serum is more stable than niosomal serum and arbutin serum. Irritant tests have shown that serum niosomes, serum transfersomes, niosomes, transfersomes, and serum bases do ot irritate rabbit skin. The diffusion profile of niosomes serum showed the ability of serum to release the drug is 2.3 times compared to vesicle-free serum, and 5,9 times compared to transfersomes serum.