SKRINING MIKROBA PENGHASIL PROTEASE DARI MAKANAN DAN MINUMAN TRADISIONAL ASAL INDONESIA

• Main Author: Alexander
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/64334
• Institution: Institut Teknologi Bandung

Protease is enzymes that are capable of degrading protein into peptide. Screening of protease producing bacteria was clone to isolate strains which have potency for pharmacy industry. In this research, screening of bacteria was carried out from traditional food samples originated from Indonesia Le. tauco, tempe gembus, tempe benguk, cladih, tape singkong, tuak nira, and tape ketan. Traditional foods are good source of protease producing bacteria and traditional food fermenting bacteria are generally known as safe (non pathogenic). The objectives of this research were to obtain potential protease producing bacteria for the production of bioactive peptide and to characterize the produced protease. Screening was conducted by inoculating the samples on modified skim milk agar, which is nutrient agar medium enriched with skim milk. Five Gram positive protease producing bacteria were isolated as protease producing bacteria i.e. DCA2 (rod-shaped and originated from tauco) and DCA3, DCA4, DCA6, and DCA10 (cocci-shaped and from tempe benguk). Characterization of extracellular protease was done by caseionolysis method, growth in nutrient gelatin medium, and zymography. The enzyme substrate specificity was tested using casein, gelatin, and collagen. Proteases from isolates under study were able to degrade casein and gelatin substrate. Isolates DCA2 produced four types of caseinase and one type of gelatinase, DCA10 produced one type of caseinase and one type of gelatinase, DCA3 and DCA4 produced one type of gelatinase. Proteases produced by the five isolats were potential to be further investigated as enzymes for production of bioactive peptides.