EFFECT OF TEMPERATUR, TOTAL ADDITION OF METANOL CONCENTRATION, AND FERMENTATION MEDIUM ON CITRIC ACID SOLID STATE FERMENTATION METHOD USING ASPERGILLUS NIGER

• Main Author: Agnesi, Maria
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/65495
• Institution: Institut Teknologi Bandung

Citric acid is a commodity that is widely used, especially in the food industry. One way to produce citric acid is by fermentation using Aspergillus niger. Citric acid fermentation using solid state method is interesting for further research because it has various advantages, such as high yield of citric acid. To produce citric acid with high yield and concentration, fermentation operating parameter should be analyzed. The operating parameters that affect the citric acid fermentation process are fermentation temperature, methanol concentration, and the amount of fermentation medium. This study was aimed to determine the opening parameter value of solid state fermentation that resulted in high productivity of citric acid. This study also aimed to determine the interaction effects between parameters ANOVA analysis on Minitab. The results of this study showed that the fermentation temperature of 37°C, methanol concentration 2%, and fermentation medium 13 mL were the operating conditions which resulted in the highest yield and concentration of citric acid (67.17 mg/g substrate). The significant factors on the yield of citric acid were fermentation temperature, methanol concentration, the amount of fermentation medium, the interaction between the fermentation temperature factor and the methanol concentration, and the interaction between the fermentation temperature and the amount of the fermentation medium.