MULTI VALENCE CE MOF AS CATALYST IN BIO-SENSOR TO MEASURE CHOLESTEROL WITH MECHANISM COLORIMETRI: SYNTHESIS, CHARACTERIZATION, AND CALCULATION DFT

• Main Author: Fendra, Yosa
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/70464
• Institution: Institut Teknologi Bandung

High level of cholesterol and glucose within our blood leads to deadly diseases. In this research, cerium benzene-1,3-5-tricarboxylate acid (CeBTC) is synthesized to be a metal organic framework (MOF) as biosensor. MOFs have advantanges as enzyme carrier and catalyst. Cerium has haloperoxide activity due to its dual oxidation states, namely Ce3+ and Ce4+. This unique state make cerium has a potential to be a biosensor. In this research cholesterol and glucose level can be detected by cerium MOF using colorimetri method. Material characterization is done to attain good synthesis. Dopants such as Nickel (Ni) and Zinc (Zn) are given to MOF to study its role in haloperoxide activity. Absorbance level is compared to calculate its bandgap energy. CeO2 and CeO2-x are also simulated using software Vienna Ab initio Simulation Package (VASP). This simulation is based on density functional theory (DFT) and the first principle. The goals are to determine their electronic structures. The results show doped cerium MOF’s activity increases as its absorbance level higher than the pristine one. The reason is due to its different bandgap energy level. Cholesterol oxidase is also immobilized within cerium MOF to detect cholesterol and glucose’s level from biologic sample using colorimetri method.