AKTIVITAS ANTIBAKTERI MINYAK DAUN NILAM (POGOSTEMON CABLIN B.), EKSTRAK ETANOL DAN FRAKSI DAUN KELOR (MORINGA OLEIFERA L.) SERTA PENENTUAN SENYAWA AKTIF SAMPEL TERPILIH TERHADAP BAKTERI PENYEBAB JERAWAT
Acne is a chronic inflammatory disease of the hair follicles in the skin associated with the oil glands due to increased androgen-induced sebum production, changes in keratinization, inflammation, and bacterial colonization of the sacs by Propionibacterium acnes and Staphylococcus aureus. This s...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/73304 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Acne is a chronic inflammatory disease of the hair follicles in the skin associated with the oil glands due
to increased androgen-induced sebum production, changes in keratinization, inflammation, and
bacterial colonization of the sacs by Propionibacterium acnes and Staphylococcus aureus. This study
aims to determine the activity of patchouli leaf oil (Pogostemon cablin B.), ethanol extract, and fraction
moringa leaf (Moringa oleifera L.) in inhibiting and killing acne-causing bacteria Propionibacterium
acnes and Staphylococcus aureus. In addition, it also determines the class of compounds that act as
antibacterial from the selected samples. Patchouli leaf oil is obtained through a process of steam
distillation. Extraction was carried out by maceration method using 96% ethanol, then fractionated by
liquid-liquid extraction method using n-hexane, ethyl-acetate, and ethanol-water. The antibacterial
activity test was carried out qualitatively using the agar disc diffusion method and TLC-bioautography,
as well as quantitatively by determining the value of the minimum inhibitory concentration (MIC) using
the microdilution method and the minimum bactericidal concentration (MBC) using the agar diffusion
method. The ethanol extract, n-hexane fraction, and ethyl acetate fraction of moringa leaves did not
show antibacterial activity in Propionibacterium acnes ATCC 11827 and Staphylococcus aureus ATCC
6538. Patchouli leaf oil has strong antibacterial activity against Propionibacterium acnes ATCC 11827
and Staphylococcus aureus ATCC 6538 with MIC–MBC values of 312.5 µg/mL and 156.25–312.5 µg/mL.
Compounds that have antibacterial activity are thought to be sesquiterpenes.
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