ENHANCING STEVIOL GLYCOSIDE PRODUCTION STRATEGY FROM STEVIA REBAUDIANA BERTONI WITH METHYL JASMONATE ELICITOR ADDITION IN EBB-AND-FLOW BIOREACTOR
Natural sweeteners in the form of stevioside and rebaudioside-A, extracted from the leaves of Stevia rebaudiana Bertoni, have a sweetness level of 150-250 and 250- 300 times sweeter than sucrose, making them highly suitable for more efficient seedling development. In this study, in vitro shoot cu...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/78265 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Natural sweeteners in the form of stevioside and rebaudioside-A, extracted from
the leaves of Stevia rebaudiana Bertoni, have a sweetness level of 150-250 and 250-
300 times sweeter than sucrose, making them highly suitable for more efficient
seedling development. In this study, in vitro shoot culture in an ebb-and-flow
bioreactor was the preferred method for better propagation compared to
conventional methods. The objective of this research was to compare the growth
performance and yield of these sweetening compounds in S. rebaudiana under
conditions without elicitation (control) and with 10 µM methyl jasmonate (Me-J)
elicitation treatment. Growth performance was determined based on parameters
such as the increase in the number of shoots, biomass increase, and relative growth
rate. Elicitation using 10 µM Me-J showed improved growth performance based
on measurable and calculated parameters. The increase in the number of shoots in
the control and treatment groups was 2.8 and 2.9 times, respectively. The increase
in biomass in the control and treatment groups was 13 and 13.7 times, respectively.
The relative growth rate in the control and treatment groups was 0.184 and 0.189
mg.mg-1.day-1. The average stevioside content in the leaves of S. rebaudiana in the
control and treatment groups was 407.94 and 655.21 µg.g-1 dry weight (DW) of
leaves, respectively. The average Reb-A content in the leaves of S. rebaudiana in
the control and treatment groups was 437.15 and 211.63 µg.g-1 DW of leaves,
respectively. Elicitation with methyl jasmonate increased the stevioside content in
S. rebaudiana by 60.6%, but it decreased the Reb-A content by 51.6%. Overall,
steviol glycosides increased in the treatment, but it is suspected that the time
required for Me-J to support the synthesis of Reb-A from stevioside was not
achieved, resulting in stevioside being the predominant steviol glycoside observed
in the extract |
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