AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla PADA Plasmodium falciparum DAN MEKANISME KERJA PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN Dihydro-orotate Dehidrogenase (DHODH)
Malaria is one of the health problem caused by parasite Plasmodium. Eastern Indonesia has the highest Annual Parasite Incidence (API). That condition is getting worse with resistance to antimalarial drugs such as quinine, chloroquine, and artemisin. The development of science, especially P. falc...
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id-langga.907672019-11-13T04:37:15Z http://repository.unair.ac.id/90767/ AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla PADA Plasmodium falciparum DAN MEKANISME KERJA PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN Dihydro-orotate Dehidrogenase (DHODH) LIA AHYUNI MULYA, 051511133054 RS Pharmacy and materia medica Malaria is one of the health problem caused by parasite Plasmodium. Eastern Indonesia has the highest Annual Parasite Incidence (API). That condition is getting worse with resistance to antimalarial drugs such as quinine, chloroquine, and artemisin. The development of science, especially P. falciparum provides an opportunity to discover new malaria drugs with the mechanism of action at the new target site that is mitochondria. An example for the new antimalarial target sites in mitochondria are mallate quinone oxidoreductase (MQO) and dihydroorootate dehydrogenase (DHODH). This study aims to find an active extract of M. triphylla which inhibit the growth of Plasmodium falciparum by MQO and DHODH assay. This study carried out the antimalarial activity test of five Melicope triphylla leaf extracts. The extracts were n-Hexane, dichloromethane, and methanol extract were extracted by gradually, alkaloid rich-extract with acid-base extraction procces from simplicia (alkaloid fraction). Those extracts were tested for their antimarial activity by three methods (microscopic method , MQO, and DHODH). Antimalarial activity with microscopic method showed that three extracts had a very active category, namely n-hexane extract (Inhibitory Concentration 50%, IC50: 0.02 μg/ml); methanol extract (IC50: 0.02 μg/ml); and dichloromethane extract (IC50: 0.08 μg/ml). Alkaloid fraction had a moderate category (IC50: 1.35 μg/ml). Antimalarial activity by MQO method showed that n-hexane extract was the most potent extract (IC50: 7.49 μg/ml). Meanwhile, antimalarial activity by DHODH method showed that methanol extract was the most potent extract (IC50: 16.37 μg/ml), and the other extract had IC50 > 100 μg/ml. The n-hexane extract inhibits MQO, but less active in inhibiting the DHODH enzyme. Therefore, all extracts need to be followed up with toxicity test (MTT) to determine the safety of the extract. 2019 Thesis NonPeerReviewed text en http://repository.unair.ac.id/90767/1/FF.FT.24-19%20Mul%20a%20abstrak.pdf text en http://repository.unair.ac.id/90767/2/FF.FT.24-19%20Mul%20a%20daftar%20isi.pdf text en http://repository.unair.ac.id/90767/3/FF.FT.24-19%20Mul%20a%20daftar%20pustaka.pdf text id http://repository.unair.ac.id/90767/4/FF.FT.24-19%20Mul%20a.pdf LIA AHYUNI MULYA, 051511133054 (2019) AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla PADA Plasmodium falciparum DAN MEKANISME KERJA PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN Dihydro-orotate Dehidrogenase (DHODH). Skripsi thesis, Universitas Airlangga. Http://lib.Unair.ac.id |
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RS Pharmacy and materia medica LIA AHYUNI MULYA, 051511133054 AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla PADA Plasmodium falciparum DAN MEKANISME KERJA PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN Dihydro-orotate Dehidrogenase (DHODH) |
description |
Malaria is one of the health problem caused by parasite
Plasmodium. Eastern Indonesia has the highest Annual Parasite Incidence
(API). That condition is getting worse with resistance to antimalarial drugs
such as quinine, chloroquine, and artemisin. The development of science,
especially P. falciparum provides an opportunity to discover new malaria
drugs with the mechanism of action at the new target site that is
mitochondria. An example for the new antimalarial target sites in
mitochondria are mallate quinone oxidoreductase (MQO) and dihydroorootate
dehydrogenase (DHODH). This study aims to find an active extract
of M. triphylla which inhibit the growth of Plasmodium falciparum by
MQO and DHODH assay. This study carried out the antimalarial activity
test of five Melicope triphylla leaf extracts. The extracts were n-Hexane,
dichloromethane, and methanol extract were extracted by gradually, alkaloid
rich-extract with acid-base extraction procces from simplicia (alkaloid
fraction). Those extracts were tested for their antimarial activity by three
methods (microscopic method , MQO, and DHODH). Antimalarial activity
with microscopic method showed that three extracts had a very active
category, namely n-hexane extract (Inhibitory Concentration 50%, IC50:
0.02 μg/ml); methanol extract (IC50: 0.02 μg/ml); and dichloromethane
extract (IC50: 0.08 μg/ml). Alkaloid fraction had a moderate category (IC50:
1.35 μg/ml). Antimalarial activity by MQO method showed that n-hexane
extract was the most potent extract (IC50: 7.49 μg/ml). Meanwhile,
antimalarial activity by DHODH method showed that methanol extract was
the most potent extract (IC50: 16.37 μg/ml), and the other extract had IC50 >
100 μg/ml. The n-hexane extract inhibits MQO, but less active in inhibiting
the DHODH enzyme. Therefore, all extracts need to be followed up with
toxicity test (MTT) to determine the safety of the extract. |
format |
Theses and Dissertations NonPeerReviewed |
author |
LIA AHYUNI MULYA, 051511133054 |
author_facet |
LIA AHYUNI MULYA, 051511133054 |
author_sort |
LIA AHYUNI MULYA, 051511133054 |
title |
AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla
PADA Plasmodium falciparum DAN MEKANISME KERJA
PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN
Dihydro-orotate Dehidrogenase (DHODH) |
title_short |
AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla
PADA Plasmodium falciparum DAN MEKANISME KERJA
PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN
Dihydro-orotate Dehidrogenase (DHODH) |
title_full |
AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla
PADA Plasmodium falciparum DAN MEKANISME KERJA
PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN
Dihydro-orotate Dehidrogenase (DHODH) |
title_fullStr |
AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla
PADA Plasmodium falciparum DAN MEKANISME KERJA
PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN
Dihydro-orotate Dehidrogenase (DHODH) |
title_full_unstemmed |
AKTIVITAS In vitro ANTIMALARIA DAUN Melicope triphylla
PADA Plasmodium falciparum DAN MEKANISME KERJA
PADA ENZIM Mallate Quinone Oxidoreductase (MQO) DAN
Dihydro-orotate Dehidrogenase (DHODH) |
title_sort |
aktivitas in vitro antimalaria daun melicope triphylla
pada plasmodium falciparum dan mekanisme kerja
pada enzim mallate quinone oxidoreductase (mqo) dan
dihydro-orotate dehidrogenase (dhodh) |
publishDate |
2019 |
url |
http://repository.unair.ac.id/90767/1/FF.FT.24-19%20Mul%20a%20abstrak.pdf http://repository.unair.ac.id/90767/2/FF.FT.24-19%20Mul%20a%20daftar%20isi.pdf http://repository.unair.ac.id/90767/3/FF.FT.24-19%20Mul%20a%20daftar%20pustaka.pdf http://repository.unair.ac.id/90767/4/FF.FT.24-19%20Mul%20a.pdf http://repository.unair.ac.id/90767/ |
_version_ |
1681152883114901504 |