PENGARUH PEMBERIAN DAGING WAGYU TERHADAP KADAR LDL, HDL, KOLESTEROL TOTAL DAN TRIGLISERIDA PADA HEWAN PERCOBAAN (TIKUS GALUR SPRAGUE DAWLEY)

• Main Authors: , OKKY LUPITA SARI, , Fatma Zuhrotun Nisaâ��, STP, MP
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/121822/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61920
• Institution: Universitas Gadjah Mada

Introduction : Dyslipidemia is a disruption or changes in blood lipid levels, include increased of total cholesterol levels or hypercholesterolemia, decreased of High Density Lipoprotein (HDL) levels, increased of Low Density Lipoprotein (LDL) levels, increased of triglyceride levels in the blood or hypertriglyceride. Replacement of saturated fatty acids with unsaturated fatty acids in the diet can decrease total cholesterol level and LDL without decrease HDL levels so as to reduce the risk of coronary heart disease. Wagyu (Japanese beef term) refers to the offspring of cattle that causes marbling effects and the production of a high percentage of unsaturated fat. Meat from wagyu cattle is known worldwide because of the marbling�s characteristics, increased eating quality through improved natural flavor, tenderness and juice levels and high market value. Wagyu cattle gene cause results on beef containing a high percentage of omega 3 and omega 6 than any other beef. Objective : To know the effect of wagyu beef on LDL levels, HDL, total cholesterol and triglyceride Sprague dawley rats. Method : The type of this research is experimental with pre test-post test and control group design in laboratorium. The subject of this research is 18 Sprague dawley rats, were divided into three groups that have the specifications of the male, age 60 days, weight 200 grams and are in good health (active and not disabled). Adaptation is performed for 7 days, after that the rats LDL levels, HDL, total cholesterol and triglyceride are checked early. Then the rats were fed a high-fat diet for 23 days. Then the LDL levels, HDL, total cholesterol and triglyceride are checked again. Later the rats were divided into 3 groups of 6 each rats, control group were fed only with the standard AIN- 93, treatment group were fed with standard AIN-93 and wagyu beef as much as 2 g/200 g weight and treatment group were fed with standard AIN-93 and wagyu beef as much as 4 g/200 g weight. Then LDL levels, HDL, total cholesterol and triglyceride were checked as the final result. Result : Adding wagyu beef in Sprague dawley rats with a dose of 4 g/200 g weight effects on decreasing total cholesterol levels (p > 0,05), increasing LDL levels (p > 0,05), decreasing HDL levels (p < 0,05) and increasing triglyceride (p > 0,05). Adding wagyu beef with a dose of 2 g/200 g weight effects on increasing total cholesterol levels (p > 0,05), increasing LDL levels (p > 0,05), increasing HDL levels (p > 0,05) and increasing triglyceride (p > 0,05). Conclusion : Wagyu beef can decrease total cholesterol levels and increase HDL levels in Sprague dawley rats.