KOMBINASI SPEKTROFOTOMETRI INFRAMERAH DAN KALIBRASI MULTIVARIAT UNTUK PENETAPAN KADAR RIFAMPISIN, ISONIAZID, PIRAZINAMID DAN ETAMBUTOL HIDROKLORIDA DALAM SEDIAAN MULTIKOMPONEN 4FDC
Some analytical techniques used for the simultaneous determination of two or more active substances are carried out using chromatographic technique, which requires high cost and time consuming, making it unfavourable in routine application. Rifampicin is unstable in solution which becomes a problem...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/133881/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=74766 |
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| Summary: | Some analytical techniques used for the simultaneous determination of
two or more active substances are carried out using chromatographic technique,
which requires high cost and time consuming, making it unfavourable in routine
application. Rifampicin is unstable in solution which becomes a problem in
analysis with chromatography or spectrophotometry. This study aimed to develop
a fast, inexpensive, simple, and reliable method for the determination of
rifampicin (RIF), isoniazid (INH), pyrazinamide (PZA) dan ethambutol
hydrochloride (ETH) based on infrared spectrophotometry in combination with
multivariate calibrations, especially partial least square (PLS). The utility of
chemometrics-assisted infrared spectrophotometry based on PLS for
determination of drug combinations has been developed.
Analysis of active component using infrared spectrophotometry-PLS is
carry out in three phases, namely calibration, validation and sample assay.
Calibration model is evaluated by the value of root mean square error of
calibration (RMSEC), root mean square error of cross validation (RMSECV), root
mean square error of prediction (RMSEP) and predicted error sum of square
(PRESS) as precision criteria and coefficient of determination (R2) as accuracy
criteria.
Results of the study showed that multivariate calibration-assisted infrared
spectrophotometry based on PLS can be used for determination of rifampicin,
isoniazid, pyrazinamide dan ethambutol hydrochloride. The optimized
wavenumber range for determination of RIF contents was 433 � 1552 cm-1 and
1756 � 3412 cm-1, 433 � 873 cm-1 for INH, 1714 � 2756 cm-1 for PZA, and 1552 �
2970 cm-1 for ETH. The PRESS values of RIF, INH, PZA and ETH contents was
0,00392%, 0,011724%, 0,007604% and 0,003368% respectively. RMSEP values
of RIF, INH, PZA and ETH contents was 0,01979%, 0,03424%, 0,027575% and
0,018351% respectively. The value of R2 was 0,990, 0,977, 0,992 and 0,998 for
RIF, INH, PZA and ETH respectively. |
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