IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
One of the modes of spread of avian influenza virus (AIV) is through distributin and marketing of poultry products in traditional markets. In relation to the marketing patten of various species oof poultry in the traditional markets, which are as live poultry and collected from different areas and f...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2012
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| Online Access: | https://repository.ugm.ac.id/97913/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54397 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | One of the modes of spread of avian influenza virus (AIV) is through
distributin and marketing of poultry products in traditional markets. In relation to
the marketing patten of various species oof poultry in the traditional markets,
which are as live poultry and collected from different areas and farms. In addition,
the whole process of poultry slaughtering to final product of carcass are in the
same location without applaying proper biosecurity. These practices will make
traditional markets as a potential source of AIV transmissios for poultry and
possibly human.
This study was designed to evaluated the critical points of AIV
contamination in traditional markets of the District of Aceh Besar of Aceh
Province, including live poultry, temporary cages, plece for slaughtering poultry
and selling carcass, and to identify the AIV subtipe in various traditional markets
in the District Aceh Besar of Aceh Province. Sampel of swabs were randomly
collected from three traditional markets in the District of Aceh Besar of Aceh
Province, namely Lambaro market, Keutapang market, and Ulekareng market,
then those sampels were pooled. Each pool of sampel contain one to five swabs,
which were arranged according to the poultry trader, species of poultry, and the
critical poins for AIV contamination. The total number of swabs were 258,
included tracheal swabs from live poultry, temporary cages, tables for poultry
slaughtering and selling carcass. Then, those swabs were grouped into 90 pools.
Isolation of AIV was performed in the chiken embryonated eggs, serologic test
was employing HA/HI methods, and molecular identification of this virus was
tested using RT-PCR method. All test were conducted at the Laboratory of
Virology and Biotechnology Center for Veterinary (BBVet) Wates. The results of
serologyc test and molecular identificatin of AIV were analyzed descriptively.
Results of this study indicated that contamination of AIV in the
traditional markets in the District of Aceh Besar and antibody against AIV were
found only ducks and broilers in the Lambaro Market. Based on RT-PCR test
employing specific primers against genes M, H5, and N1, it was concluded that
AIV that has beeen circulating in the Lambaro Market is H5N1 subtype. Results
of this reseach proved that among several critical points of AIV contamination in
traditional market, live poultry (ducks and broilers) which are kept in temporary
cages can act as a potential source of AIV spreading into the environment. |
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