IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH

One of the modes of spread of avian influenza virus (AIV) is through distributin and marketing of poultry products in traditional markets. In relation to the marketing patten of various species oof poultry in the traditional markets, which are as live poultry and collected from different areas and f...

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Main Authors: , drh.Rika Yulisma, , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D
Format: Theses and Dissertations NonPeerReviewed
Published: [Yogyakarta] : Universitas Gadjah Mada 2012
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ETD
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spelling id-ugm-repo.979132016-03-04T08:49:43Z https://repository.ugm.ac.id/97913/ IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH , drh.Rika Yulisma , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D ETD One of the modes of spread of avian influenza virus (AIV) is through distributin and marketing of poultry products in traditional markets. In relation to the marketing patten of various species oof poultry in the traditional markets, which are as live poultry and collected from different areas and farms. In addition, the whole process of poultry slaughtering to final product of carcass are in the same location without applaying proper biosecurity. These practices will make traditional markets as a potential source of AIV transmissios for poultry and possibly human. This study was designed to evaluated the critical points of AIV contamination in traditional markets of the District of Aceh Besar of Aceh Province, including live poultry, temporary cages, plece for slaughtering poultry and selling carcass, and to identify the AIV subtipe in various traditional markets in the District Aceh Besar of Aceh Province. Sampel of swabs were randomly collected from three traditional markets in the District of Aceh Besar of Aceh Province, namely Lambaro market, Keutapang market, and Ulekareng market, then those sampels were pooled. Each pool of sampel contain one to five swabs, which were arranged according to the poultry trader, species of poultry, and the critical poins for AIV contamination. The total number of swabs were 258, included tracheal swabs from live poultry, temporary cages, tables for poultry slaughtering and selling carcass. Then, those swabs were grouped into 90 pools. Isolation of AIV was performed in the chiken embryonated eggs, serologic test was employing HA/HI methods, and molecular identification of this virus was tested using RT-PCR method. All test were conducted at the Laboratory of Virology and Biotechnology Center for Veterinary (BBVet) Wates. The results of serologyc test and molecular identificatin of AIV were analyzed descriptively. Results of this study indicated that contamination of AIV in the traditional markets in the District of Aceh Besar and antibody against AIV were found only ducks and broilers in the Lambaro Market. Based on RT-PCR test employing specific primers against genes M, H5, and N1, it was concluded that AIV that has beeen circulating in the Lambaro Market is H5N1 subtype. Results of this reseach proved that among several critical points of AIV contamination in traditional market, live poultry (ducks and broilers) which are kept in temporary cages can act as a potential source of AIV spreading into the environment. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , drh.Rika Yulisma and , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D (2012) IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54397
institution Universitas Gadjah Mada
building UGM Library
country Indonesia
collection Repository Civitas UGM
topic ETD
spellingShingle ETD
, drh.Rika Yulisma
, Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D
IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
description One of the modes of spread of avian influenza virus (AIV) is through distributin and marketing of poultry products in traditional markets. In relation to the marketing patten of various species oof poultry in the traditional markets, which are as live poultry and collected from different areas and farms. In addition, the whole process of poultry slaughtering to final product of carcass are in the same location without applaying proper biosecurity. These practices will make traditional markets as a potential source of AIV transmissios for poultry and possibly human. This study was designed to evaluated the critical points of AIV contamination in traditional markets of the District of Aceh Besar of Aceh Province, including live poultry, temporary cages, plece for slaughtering poultry and selling carcass, and to identify the AIV subtipe in various traditional markets in the District Aceh Besar of Aceh Province. Sampel of swabs were randomly collected from three traditional markets in the District of Aceh Besar of Aceh Province, namely Lambaro market, Keutapang market, and Ulekareng market, then those sampels were pooled. Each pool of sampel contain one to five swabs, which were arranged according to the poultry trader, species of poultry, and the critical poins for AIV contamination. The total number of swabs were 258, included tracheal swabs from live poultry, temporary cages, tables for poultry slaughtering and selling carcass. Then, those swabs were grouped into 90 pools. Isolation of AIV was performed in the chiken embryonated eggs, serologic test was employing HA/HI methods, and molecular identification of this virus was tested using RT-PCR method. All test were conducted at the Laboratory of Virology and Biotechnology Center for Veterinary (BBVet) Wates. The results of serologyc test and molecular identificatin of AIV were analyzed descriptively. Results of this study indicated that contamination of AIV in the traditional markets in the District of Aceh Besar and antibody against AIV were found only ducks and broilers in the Lambaro Market. Based on RT-PCR test employing specific primers against genes M, H5, and N1, it was concluded that AIV that has beeen circulating in the Lambaro Market is H5N1 subtype. Results of this reseach proved that among several critical points of AIV contamination in traditional market, live poultry (ducks and broilers) which are kept in temporary cages can act as a potential source of AIV spreading into the environment.
format Theses and Dissertations
NonPeerReviewed
author , drh.Rika Yulisma
, Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D
author_facet , drh.Rika Yulisma
, Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D
author_sort , drh.Rika Yulisma
title IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
title_short IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
title_full IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
title_fullStr IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
title_full_unstemmed IDENTIFIKASI CEMARAN VIRUS AVIAN INFLUENZA DI PASAR TRADISIONAL KABUPATEN ACEH BESAR PROVINSI ACEH
title_sort identifikasi cemaran virus avian influenza di pasar tradisional kabupaten aceh besar provinsi aceh
publisher [Yogyakarta] : Universitas Gadjah Mada
publishDate 2012
url https://repository.ugm.ac.id/97913/
http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54397
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