Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and malt extracts as the carbon and nitrogen sources, respectively, produced the greatest amount of mycelium. Sources that produced th...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/2875/1/FH02-FBIM-16-06625.pdf http://eprints.unisza.edu.my/2875/ |
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| Institution: | Universiti Sultan Zainal Abidin |
| Language: | English |
| Summary: | The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the
growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and
malt extracts as the carbon and nitrogen sources, respectively, produced the
greatest amount of mycelium. Sources that produced the most biomass were
chosen as carbon and nitrogen sources in a C:N ratio test. However, in further
experiments, glucose was chosen as the carbon source for the C:N ratio test
because rice flour was found to be easily contaminated. Under the C:N ratio test
(fixed carbon test), the highest spore production was obtained with a 5:1 ratio (4.78
× 106 spores/mL) and the highest biomass production was obtained with a 7.5:1
ratio (4.66 g/100 mL). In the fixed nitrogen test, 7.5:1 ratio provided the greatest
output (4.08 × 106 spores/mL) whereas a 5:1 ratio produced the most biomass
(4.33 g/100 mL). Meanwhile, the control which consisted of V8 agar without
additional carbon and nitrogen source produced 1.07 × 106 spores/mL. These
results provide information on the influence of carbon and nitrogen source and the
C:N ratio that can be used in media for optimum growth and spore yield |
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