Total phenolics contents and free radical scavenging capacity of piper sarmentosum (pucuk kaduk) extract
Introduction: Piper sarmentosum is one of the herbaceous plants that has been used as natural antioxidant to source to treat diseases. This study was conducted to determine the total phenolic contents (TPC) and free radical scavenging capacity in free and bound (soluble and insoluble) of P. sa...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
International Islamic University Malaysia
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/110796/7/110796_Total%20phenolics%20contents%20and%20free%20radical%20scavenging.pdf http://irep.iium.edu.my/110796/ https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/184 |
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| Institution: | Universiti Islam Antarabangsa Malaysia |
| Language: | English |
| Summary: | Introduction: Piper sarmentosum is one of the herbaceous plants that has been used as natural
antioxidant to source to treat diseases. This study was conducted to determine the total phenolic
contents (TPC) and free radical scavenging capacity in free and bound (soluble and insoluble) of P.
sarmentosum. Methods: Free phenolic extract was acquired through direct methanol extraction while
acidic and alkaline hydrolyses were adopted to release the bound phenolic acids. The TPC was
determined by using Folin-Ciocalteu assay and is expressed as Gallic Acid equivalent (GAE) in
miligrams per gram of extracts. The antioxidant scavenging capacity was determined by using DPPH
(2, 2-diphenyl-1-picrylhydrazyl) assay. Results: Insoluble bound phenolic extract of P. sarmentosum
showed the highest TPC value (1.54 ± 0.04 mg GAE/g DW) followed by soluble phenolic extract and
free extract (1.13 ± 0.10 and 0.57 ± 0.06 mg GAE/g DW, respectively). The soluble phenolic fraction has
expressed the highest free radical scavenging capacity (76.57± 4.12%) followed by insoluble (69.79± 2.33
%) and free extracts (58.15± 4.44 %). The IC50 values for free, soluble and insoluble bound phenolic were
24.05 ± 3.81, 16.17 ± 1.84 and 18.49 ± 1.92 mg/ml, respectively. Conclusions: The significant differences
between all the extracts and antioxidant inhibition in this present study suggested that different forms
(free and bound) of extracts did influence the radical scavenging capacity as a whole. |
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