Escherichia coli glucuronylsynthase: an engineered enzyme for the synthesis of β-glucuronides

• Main Authors: Wilkinson, Shane M., Liew, Chu W., Mackay, Joel P., Mohd. Salleh, Hamzah, Withers, Stephen G., McLeod, Malcolm D.
• Format: Article
• Language: English
• Published: American Chemical Society 2008
• Subjects: T175 Industrial research. Research and development; TA164 Bioengineering; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/23428/1/Organic_letters_2008.pdf; http://irep.iium.edu.my/23428/; http://pubs.acs.org/doi/abs/10.1021/ol8002767?journalCode=orlef7
• Institution: Universiti Islam Antarabangsa Malaysia

The glycosynthase derived from E. coli β-glucuronidase catalyzes the glucuronylation of a range of primary, secondary, and aryl alcohols with moderate to excellent yields. The procedure provides an efficient, stereoselective, and scalable single-step synthesis of β-glucuronides under mild conditions...