Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH v...

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Bibliographic Details
Main Authors: Lin, Y.K., Show, P.L., Yap, Y.J., Tan, C.P., Ng, E.P., Ariff, A.B., Annuar, M.S.M., Ling, T.C.
Format: Article
Published: 2015
Subjects:
QR Microbiology
Online Access:http://eprints.um.edu.my/16468/
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Institution: Universiti Malaya
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Summary:Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, V-R of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (P-FT) of 21.8 and a yield (Y-T) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.

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