Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH v...

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Main Authors: Lin, Y.K., Show, P.L., Yap, Y.J., Tan, C.P., Ng, E.P., Ariff, A.B., Annuar, M.S.M., Ling, T.C.
Format: Article
Published: 2015
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QR Microbiology
Online Access:http://eprints.um.edu.my/16468/
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Institution: Universiti Malaya
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spelling my.um.eprints.164682016-09-15T08:06:06Z http://eprints.um.edu.my/16468/ Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation Lin, Y.K. Show, P.L. Yap, Y.J. Tan, C.P. Ng, E.P. Ariff, A.B. Annuar, M.S.M. Ling, T.C. QR Microbiology Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, V-R of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (P-FT) of 21.8 and a yield (Y-T) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF. (c) 2015, The Society for Biotechnology, Japan. All rights reserved. 2015 Article PeerReviewed Lin, Y.K. and Show, P.L. and Yap, Y.J. and Tan, C.P. and Ng, E.P. and Ariff, A.B. and Annuar, M.S.M. and Ling, T.C. (2015) Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation. Journal of Bioscience and Bioengineering , 120 (6). pp. 684-689. ISSN 1389-1723 DOI: 10.1016/j.jbiosc.2015.04.013
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QR Microbiology
spellingShingle QR Microbiology
Lin, Y.K.
Show, P.L.
Yap, Y.J.
Tan, C.P.
Ng, E.P.
Ariff, A.B.
Annuar, M.S.M.
Ling, T.C.
Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
description Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, V-R of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (P-FT) of 21.8 and a yield (Y-T) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.
format Article
author Lin, Y.K.
Show, P.L.
Yap, Y.J.
Tan, C.P.
Ng, E.P.
Ariff, A.B.
Annuar, M.S.M.
Ling, T.C.
author_facet Lin, Y.K.
Show, P.L.
Yap, Y.J.
Tan, C.P.
Ng, E.P.
Ariff, A.B.
Annuar, M.S.M.
Ling, T.C.
author_sort Lin, Y.K.
title Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
title_short Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
title_full Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
title_fullStr Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
title_full_unstemmed Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
title_sort direct recovery of cyclodextringlycosyltransferase from bacillus cereus using aqueous two-phase flotation
publishDate 2015
url http://eprints.um.edu.my/16468/
_version_ 1643690282674290688

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