Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

• Main Authors: Lin, Y.K., Show, P.L., Yap, Y.J., Tan, C.P., Ng, E.P., Ariff, A.B., Annuar, M.S.M., Ling, T.C.
• Format: Article
• Published: 2015
• Subjects: QR Microbiology
• Online Access: http://eprints.um.edu.my/16468/
• Institution: Universiti Malaya

Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH v...