A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3

A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3

Although the crystal structure of DEN2 NS3 serine protease has been reported, the proteolytic mechanism of this enzyme in the presence of NS2B as co-factor which greatly enhances its activity is not well-understood. Using the homology model of DEN2 NS3 co-complexed with NS2B co-factor based on HCV N...

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Main Authors: Lee, Y.K., Othman, R., Abdul Wahab, H., Yusof, Rohana, Rahman, N.A.
Format: Article
Published: Faculty of Science, University of Malaya 2006
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/7139/
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spelling my.um.eprints.71392021-04-28T06:41:58Z http://eprints.um.edu.my/7139/ A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3 Lee, Y.K. Othman, R. Abdul Wahab, H. Yusof, Rohana Rahman, N.A. R Medicine Although the crystal structure of DEN2 NS3 serine protease has been reported, the proteolytic mechanism of this enzyme in the presence of NS2B as co-factor which greatly enhances its activity is not well-understood. Using the homology model of DEN2 NS3 co-complexed with NS2B co-factor based on HCV NS3/4A as the proposed template, the model of the DEN2 NS2B/3 complex was reproduced and its structure evaluated through PROCHECK, VERIFY3D and ERRAT. Comparison of the homology model with the crystal structure of DEN2 NS3 revealed that this homology model served as a better choice for the NS2B/3 protein model in drug design. Faculty of Science, University of Malaya 2006 Article PeerReviewed Lee, Y.K. and Othman, R. and Abdul Wahab, H. and Yusof, Rohana and Rahman, N.A. (2006) A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3. Malaysian Journal of Science, 25. pp. 15-22. ISSN 1394-3065
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Lee, Y.K.
Othman, R.
Abdul Wahab, H.
Yusof, Rohana
Rahman, N.A.
A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3
description Although the crystal structure of DEN2 NS3 serine protease has been reported, the proteolytic mechanism of this enzyme in the presence of NS2B as co-factor which greatly enhances its activity is not well-understood. Using the homology model of DEN2 NS3 co-complexed with NS2B co-factor based on HCV NS3/4A as the proposed template, the model of the DEN2 NS2B/3 complex was reproduced and its structure evaluated through PROCHECK, VERIFY3D and ERRAT. Comparison of the homology model with the crystal structure of DEN2 NS3 revealed that this homology model served as a better choice for the NS2B/3 protein model in drug design.
format Article
author Lee, Y.K.
Othman, R.
Abdul Wahab, H.
Yusof, Rohana
Rahman, N.A.
author_facet Lee, Y.K.
Othman, R.
Abdul Wahab, H.
Yusof, Rohana
Rahman, N.A.
author_sort Lee, Y.K.
title A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3
title_short A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3
title_full A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3
title_fullStr A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3
title_full_unstemmed A revisit into the Den2 NS2B/NS3 virus protease homology model: Structural verification and comparison with crystal structure of HCV NS3/4A and Den2 NS3
title_sort revisit into the den2 ns2b/ns3 virus protease homology model: structural verification and comparison with crystal structure of hcv ns3/4a and den2 ns3
publisher Faculty of Science, University of Malaya
publishDate 2006
url http://eprints.um.edu.my/7139/
_version_ 1698697304414879744

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